Effects of nerve growth factor-polybutylcyanoacrylate-nanoparticles on in vitro cellular models of Alzheimer's disease
- Author:
- No author available
- Journal Title:
- Chinese Journal of Neuromedicine
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2012.03.005
- Key Word:
- 阿尔茨海默病;神经生长因子;聚氰基丙烯酸丁酯纳米粒;肾上腺嗜铬细胞瘤细胞;Nerve growth factor;Alzheimer's disease;Polybutylcyanoacrylate-nanoparticles;PC12
Abstract： Objective To evaluate the effects of nerve growth factor (NGF)polybutylcyanoacrylate (PBCA)- nanoparticles (NP) on PC12 cells induced by Aβ1-40 Methods The emulsion polymerization method was used to prepare the NGF-PBCA-NP. Morphology of the NGF-PBCA-NP was observed by transmission electron microscopy. The entrapment efficiency and loading efficiency of NGF-PBCA-NP were measured by means of ultraviolet spectrophotometry. The effects of NGF-PBCA-NP on proliferation and cell apoptosis of β1-40-PC12 cells were observed by MTT and FCM methods. Results The NGF-PBCA-NPs were uniform spheres with drug entrapment efficiency of 80.87％ and loading efficiency of 19.88％.Compared with the cells in the NGF group,cells in the NGF-PBCA-NP group exhibited a significantly better proliferation rate and a significantly lower apoptosis rate (P＜0.05). Conclusion NGF-PBCA-NPs obtained by emulsion polymerization method are uniform in morphology and possess a high drug entrapment efficiency and a high loading efficiency.They can restrain significantly the in vitro growth of Aβ1-40-PC12.
- [1]穆俊林,宋景贵,张宁,等.纳米化苯妥英钠抗大鼠癫痫模型疗效的研究[J].2011,(4).
- [2]布林,王东军,钟环,等.肋间神经移植加NGF、BDNF基因修饰的OECs修复大鼠脊髓损伤[J].2006,(4).
- [3]樊俊,吕延成.纳米粒载体在小RNA干扰技术中的研究进展[J].2010,(4).
- [4]李晶,汪森明,胡丽娟,等.盐酸洛拉曲克壳聚糖纳米粒的制备及其特性研究[J].2011,(9).
- [5]Maki M,Kuzmin Nichols N,Hayashi T,etc.Mannitol facilitates neurotrophic factor up-regulation and behavioural recovery in neonatal hypoxic-ischaemic rats with human umbilical cord blood grafts.[J].2010,14(4).
- [6]Kreuter, J.,Khalansky, A.S.,Wohlfart, S.,etc.Kinetics of transport of doxorubicin bound to nanoparticles across the blood-brain barrier[J].2011,154(1).
- [7]Pinol Ripoll,Ilieva EV,Kichev A.Cell death and learning impairment in mice caused by in vitro modified pro-NGF can be related to its increased oxidative modifications in Alzheimer disease.[J].2009,175(6).
- [8]Gu H,Song C,Long D,etc.Recombinant human NGF-loaded microspheres promote survival of basal forebrain cholinergic neurons and improve memory impairments of spatial learning in the rat model of Alzheimer's disease with fimbria-fornix lesion.[J].2009,453(3).