Effect of Shenfu injection on expressions of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1 in rats after cerebral ischemia-reperfnsion injury

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Author:
JIANG Cheng-ping(Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007,China)
LIU Fu(Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007,China)
LI Yi(Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007,China)
WANG Bai-qiang(Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007,China)
TANG Xiao-rong(Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007,China)
WU Bi-hua()
WANG Xiao-ming()
Journal Title:
Chinese Journal of Neuromedicine
Issue:
Volume 11, Issue 01, 2012
DOI:
10.3760/cma.j.issn.1671-8925.2012.01.005
Key Word:
Shenfu injection;Cerebral ischemia/reperfusion;Brain edema;Matrix metalloproteinase-9

Abstract: Objective To investigate the effect of Shenfu injection on the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in rats after cerebral ischemia-reperfusion injury,and explore the brain protection mechanism of Shenfu injection. Methods Ninety adult healthy male Wister rats were chosen and randomly divided into sham-operated group,Shenfu injection treatment group and vehicle group (n=30); animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery.Rats in the Shenfu injection treatment group were injected with 8 mg/kg Shenfu injection during the reperfusion while those in the vehicle group with physiological saline.These rats in each group were divided into 3 sub-groups (n=10) according to the ischemia time (0.5,1 and 1.5 h after the model establishment). Twenty-four h after reperfusion, dry-weight method was employed to detect the content of brain tissues on the side received ischemia-reperfusion; and Western blotting was employed to observe the expressions of MMP-9 and TIMP-1 in the brain tissues. Results The water content of those brain tissues and the expressions of MMP-9 and TIMP-1 in the Shenfu injection treatment group and vehicle group at each time point significantly increased as compared with those in the sham-operated group (P<0.05); the longer the ischemic time,the heavier the water content of the brain tissues and the higher the expressions of MMP-9 and TIMP-1. As compared with those in the vehicle group, the water content of the brain tissues and the expression of MMP-9 in the Shenfu injection treatment group obviously decreased,while the expression of TIMP-1 in the Shenfu injection treatment group significantly increased at each time point (P<0.05). Conclusion Shenfu injection has the function against cerebral edema by accommodating the expressions of MMP-9 and TIMP-1.

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