Transforming growth factor β2 mRNA expression in human brain glioma stem cells and its significance
- Author:
- No author available
- Journal Title:
- Chinese Journal of Neuromedicine
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-8925.2012.01.003
- Key Word:
- 神经胶质瘤;脑胶质瘤干细胞;转化生长因子-β2;免疫治疗;Glioma;Brain glioma stem cell;Transforming growth factor β2;Immunotherapy
Abstract： Objective To determine the mRNA expression of transforming growth factor β2 (TGF-β2) in human brain glioma stem cells (BGSCs),and investigate its effect onimmunotherapy of glioma. Methods BGSCs were grown from 8 specimens resected from glioma from October 2008 to January 2009,then,they were isolated and identified.The expressions ofCD133,GFAP and TU-20 in the tumor cell spheres were detected by immunofluorescence staining; the TGF-β2 mRNA expression in BGSCs was detected by RT-PCR,and then,this result was compared with that of respective differentiated glioma cells. Results Positive expression of CD133 was noted in the tumor cell spheres,and GFAP and TU-20 could be expressed in the tumor cell spheres. A quantitative determination exhibited higher TGF-β2 mRNA expression in BGSCs than that in their counterparts of primary cultured glioma cells,which was statistically significant (t=3.619,P=0.009). Conclusion High TGF-β2 mRNA expression is noted in BGSCs,which might be the main producer of overproduction of TGF-β2 in gliomas leading to immune escape and resistance to current therapies.
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