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Establishment of incomplete spinal cord ischemic injury rabbit models and their significance

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Author:
No author available
Journal Title:
Chinese Journal of Neuromedicine
Issue:
10
DOI:
10.3760/cma.j.issn.1671-8925.2011.10.005
Key Word:
脊髓损伤;动物模型;腰动脉;Spinal cord injury;Animal model;Lumbar

Abstract: Objective To explore the establishment of animal models of incomplete spinal cord ischemic injury to provide ideal carriers for researching the pathogenesis of incomplete spinal cord ischemic injury.Methods Twenty-four New Zealand rabbits were randomly divided into a control group (n=8) and 2 experimental groups (n=16); control group only underwent sham-operation without inducing spinal cord injury to exclude the influences of anesthesia and surgery on motor evoked potential (MEP); in the experimental groups,spinal cord ischemia injury models were established by the methods of selective ligation of segmental spinal artery from cranio-caudal direction.Baseline MEP after anesthetization were recorded,and the MEP 30 min,2 and 7 d after vascular ligation were noted.Motor function was assessed after narcotic conscious,and 2 and 7 d after vascular ligation.The specimens 7 d after ligation were taken for HE staining.Results The amplitude of MEP in the experimental group having 3 lumbar artery ligation 30 minutes after ligation was significantly different as compared with that in the control group (P<0.05); no significant differences on the amplitude of MEP were noted between these 2 groups 2 and 7 d after ligation (P>0.05).Significant differences on the amplitude of MEP were noted between control group and experimental group having 4 lumbar artery ligation 30 min,and 2 and 7 d after ligation (P<0.05).The latency of all these rabbits showed no significant difference 30 min,and 2 and 7 d after ligation (P>0.05).The amplitude changes of MEP were accorded with the results of motor function scale.Conclusion Reversible incomplete spinal cord ischemia animal models can be established after 3 lumbar artery ligation; irreversible incomplete spinal cord ischemia animal models can be established after 4 lumbar artery ligation.

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