Degeneration of peripheral nerves and expression changes of microtubule-associated protein 1B in rats after chronic spinal cord compression

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Author:
LI Li-jun(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
SONG Jie-fu(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
WEI Jie(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
CHANG Feng(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
SU Yun-xing(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
CHANG Bao-guo(Department of Orthopaedics, Shanxi Provincial Hospital, Taiyuan 030001, China)
Journal Title:
Chinese Journal of Neuromedicine
Issue:
Volume 10, Issue 10, 2011
DOI:
10.3760/cma.j.issn.1671-8925.2011.10.004
Key Word:
Spinal cord injury;Peripheral nerve;Microtubule-associated protein;Degeneration;Axonal regeneration

Abstract: Objective To observe the morphological changes of the peripheral nerves,the expression changes of microtubule-associated protein 1B (MAP1B) and the changes of motor function in rats after chronic spinal cord compression.Methods A total of 50 female Wistar rats were randomly divided into normal control group (n=10),sham-operated group (n=10) and chronic compressive groups (n=30).The rats in the chronic compressive groups were given gradual compression on the posterior spinal cord using blunt plastics screw; compression degree reached 20% (n=10),40% (n=10) and 60% (n=10),respectively,after 2 months.Rats in the normal control group did not receive any treatment and rats in the sham-operated group was only given removal of the L5 spinous process and part of the vertebral plate.Following sacrifice of the rats,cells from sciatic nerves were removed for HE staining;light microscopy and electron microscopy were employed to observe the changes; immunohistochemical staining of MAP1B was performed.Results Hypokinesia,and decreased Tarlov scores,Ramp test scores and BBB-21 scores in the chronic compressive groups were noted as compared with those in the normal control group (P<0.05).Peripheral nerve degeneration was noted in all the chronic compressive groups; the more severe the compression,the more significant the degeneration.Expression of MAP1B in the peripheral nerves of the chronic compressive groups was significantly down-regulated as compared with that in the normal control group (P<0.05).Conclusion Spinal cord compressive injury can lead to peripheral nerve degeneration; and neuronal apoptosis and necrosis lead to rare axonal regeneration,which may be one of the important reasons that influences the neural function recovery after chronic spinal cord compression.

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