Effect of diphenylhydantoin sodium polybutylcyanoacrylate nanoparticles on rat models of epilepsy

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Author:
MU Jun-lin(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
SONG Jing-gui(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
ZHANG Ning(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
DU Hao-rui(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
LI Wen-qiang(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
WANG Xia-hong(Electrophysiology Laboratory, Second Affiliated Hospital of Xinxiang Medical College, Xinxiang 453002, China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 10, Issue 04, 2011
DOI:
10.3760/cma.j.issn.1671-8925.2011.04.002
Key Word:
Epilepsy;Nanoparticle;Diphenylhydantoin sodium;Poly n-butyl cyanoacrylate

Abstract: Objective To evaluate the effects of diphenylhydantoin sodiumpolybutylcyanoacrylate nanoparticles (DPH-PBCA-NPs) and DPH-PBCA-NPs modified with Tween-80on rat models of epilepsy, and investigate the advantage of nanoparticle as the drug delivery system.Methods The rat models of acute epilepsy induced by lithium pilocarpine were established and randomly divided into 5 groups: group Ⅰ (performing injection of DPH-PBCA-NPs modified with Tween-80), group Ⅱ (performing injection of DPH-PBCA-NPs), group Ⅲ (performing injection of diphenylhydantoin sodium), group Ⅳ (performing injection of PBCA-NPs) and group Ⅴ (performing injection of physiological saline). The changes of electroencephalogram (EEG) manifestations of these rats were observed by using video-EEG monitoring; and their behavioral changes were noted too.Results The lithium pilocarpine induced rat models of acute epilepsy were successfully established and their status epilepticus were confirmed by EEG and their behaviors. The effective rate of DPH-PBCA-NPs modified with Tween-80 and DPH-PBCA-NPs was 91.67% and 54.55%, respectively;the effective rate of rats in group Ⅲ was 50%, and the effective rate of rats in group Ⅳ and Ⅴ was 0%;DPH-PBCA-NPs modified with Tween-80 enjoyed a better effect than DPH-PBCA-NPs and DPH (P<0.05). Conclusion DPH-PBCA-NPs and DPH-PBCA-NPs modified with Tween-80 can be used to improve the behaviors of rats with acute epilepsy and modify the results of EEG of these rats.Nanoparticles as drug delivery system can help the drugs having their effects much quickly and effectively.

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