Reversal effect of single intravenous injection of erythropoietin on vasospasm in the basilar artery following subarachnoid hemorrhage
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1671-8925.2010.07.009
- Key Word:
- 促红细胞生成素;蛛网膜下腔出血;脑血管痉挛;Erythropoietin;Subarachnoid hemorrhage;Cerebral vascular
Abstract： Objective To investigate the reversal effect of intravenous injection of erythropoietin on vasospasm in the basilar artery in rabbits subjected to subarachnoid hemorrhage (SAH). Methods Thirty male New Zealand adult white rabbits weighing 2.0-2.5 kg were equally randomized into 5 groups: sham-operated group, SAH0 group, SAH1 group, SAH2 group and SAH3 group. SAH models of single-hemorrhage were established by injecting autologous arterial blood (1.0 mL/kg) into the cistema magna in SAH group's rabbits, while animals in the sham-operated group received an injection of normal saline (1.0 mlAg). Twenty-four h after SAH, rabbits in the sham-operated group and the SAHO group were received intravenous injection of normal saline (0.1 mL/kg), meanwhile, intravenous injection of r-Hu-EPO at dosages of 500,1000, or 2000 IU/kg was initiated in the SAH1 group, SAH2 group SAH3 group, respectively. All animals were sacrificed by perfusion-fixation 48 h after SAH induction. The cross section areas of basilar arteries, corrugation coefficient (CC) of the basilar arteries, and hippocampus normal neuron density of CA1 area were measured by Image-Pro-Plus software. Results Morphology observation on the basilar arteries showed no obvious changes in the sham-operated group, significantly thickened and disorganized vascular walls with thickened tunica media and disorganized smooth muscle in the SAH0 and SAH1 groups; the internal elastic lamina in the SAH3 group was folded. Compared with that in SAH0 group [(0.07±0.02) mm2], the cross-sectional area of basilar arteries in SAH2 [(0.10±0.01) mm2] and SAH3 [(0.16±0.02) mm2] groups were significantly increased (P<0.05); the CC in SAH2 (1.22± 0.06) and SAH3 (1.15±0.03) groups was significantly lower than that in the SAHO group (1.31±0.09, P<0.05); and the normal neural density of hippocampus in CA1 area in the SAH3 group [(126.8±5.7) cell/mm] was significantly higher than that in the SAHO group [(99.3±9.6) cell/mm, P<0.05]. Conclusion A single intravenous injection of erythropoietin 24 h after SAH can still reverse SAH-induced vasospasm and attenuate injury of neurons.
- [1]钱涛,张庆俊.实验性蛛网膜下腔出血后海马CA1区神经元凋亡的研究[J].2006,(2).
- [2]尹泓,薛欣盛,马永丰,等.枕大池注入促红细胞生成素在兔蛛网膜下腔出血的脑保护作用[J].2008,(4).
- [3]白万胜,高国栋,赵振伟,等.兔脑血管痉挛后海马CA1区c-FOS表达及尼莫地平的神经保护作用[J].2003,(23).
- [4]高成,刘相轸,刘巍,等.酪氨酸激酶抑制剂对大鼠蛛网膜下腔出血后脑血管痉挛的作用研究[J].2006,(3).
- [5]黄新,史继新,李劲松,等.促红细胞生成素缓解蛛网膜下腔出血后脑血管痉挛的实验研究[J].2008,(9).
- [6]王樑,高国栋,赵振伟,等.TRAIL及其受体与蛛网膜下腔出血后痉挛动脉内皮细胞的凋亡[J].2005,(2).
- [7]冯金福,段传志,李西锋,等.蛛网膜下腔出血患者迟发性脑血管痉挛相关因素的临床分析[J].2009,(5).
- [8]Keenan S,Ghezzi P,Cerami A,etc.Erythropoietin crosses the blood-brain barrier to protect against experimental brain injury.[J].2000,97(19).
- [9]Kovacic S,Bunc G,Ravnik J.Correspondence between the time course of cerebral vasospasm and the level of cerebral dopamine-beta-hydroxylase in rabbits.[J].2006,130(1).
- [10]Kovacic S,Strnad S,Bunc G.Evaluation of functional response of cerebral arteries by a new morphometric technique.[J].2001,93(1).
- [11]Buemi M,Salpietro FM,Sfacteria A,etc.Beneficial effects of systemic administration of recombinant human erythropoietin in rabbits subjected to subarachnoid hemorrhage.[J].2002,99(8).
- [12]Anantha Vijay R SANTHANAM,Zvonimir S KATUSIC.Erythropoietin and cerebral vascular protection: role of nitric oxide[J].2006,(11).
- [13]Grasso G,Aloisi C,Corica F,etc.In vivo evidence that erythropoietin has a neuroprotective effect during subarachnoid hemorrhage.[J].2000,392(1).
- [14]Wang PP,Frazier JL,Pradilla G,etc.Inhibition of cerebral vasospasm by intracranial delivery of ibuprofen from a controlled-release polymer in a rabbit model of subarachnoid hemorrhage.[J].2004,101(1).