Abstract： Objective To investigate the effect of suspended cells from the hippocampus of epileptic rats on the differentiation of hippocampal stem cells.Methods Eight groups, including control group,glutamic acid groups (5 and 25 μmol/mL groups),normal hippocampal cells and epileptic cells groups (20 and 40 μmol/mL groups,respectively) were chosen in the experiment.The suspended cells were extracted from the hippocampus of epileptic rats and normal rats,and corresponding dose of them were added into the epileptic cells groups and normal hippocampal cells groups,respectively.Five μmol/mL and 25 μmol/mL glutamic acid were added into the glutamic acid groups,respectively.The vitality of differentiated stem cells was detected and compared with MTT method.Results MTT value (the vitality of differentiated stem cells) was increased following the increased differentiation time.Cell viability in the glutamic acid groups and normal hippocampal cells groups was slightly decreased as compared with that in the control group (P>0.05).The cell viability in the epileptic cells groups was slightly increased as compared with that in the control group (P>0.05).The MTT value in the epileptic cells group and the normal hippocampal cells group was not obviously different (P>0.05).Conclusion The suspended cells from the hippocampus of epileptic rats do not affect the differentiation of the hippocampal stem cells.