Expression of TrKA in animal models with incompletely optic nerve injury and effect of local delivery of NGF on its expression

( views:, downloads: )
Author:
LIN Ning(Department of Neurosurgery,Xiehe Hospital,Huazhong University of Science and Technology,Wuhan 430022,China)
LIU Xiao(Department of Neurosurgery,Xiehe Hospital,Huazhong University of Science and Technology,Wuhan 430022,China)
ZHOU Ying-chun(Department of Neurosurgery,Xiehe Hospital,Huazhong University of Science and Technology,Wuhan 430022,China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 9, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1671-8925.2010.03.004
Key Word:
TrkA;Nerve growth factor;Optic nerve injury

Abstract: Objective To explore the expression of high-affinity NGF receptor TrkA and the effect of local delivery of NGF carried by fibrin sealant on its expression in animal models with incompletely optic nerve injury.Methods Male SD rats were randomized into injured group,NGF-treated group and normal control group.The injured models were induced by aneurysm clamp.RT-PCR was employed to detect the mRNA expression of TrkA in the 3 groups on the 3~(rd),5~(th),7~(th),10~(th) d of injury,respectively.Results The relative value in the normal control group was 0.44±0.06.The value of injured and NGF-treated groups was remarkably increased on the 3~(rd) d of injury(P<0.05).The value in the NGF-treated group was increased by 7.1,35.0,19.6 and 9.5 times of that in the control group on the 3~(rd),5~(th),7~(th) and 10~(th) d of injury,respectively;that in the injured group was 8.0,32.4,20.4 and 8.5 times,respectively;no significant difference of the relative value was noted between the 2 groups at different time points(P>0.05).Conclusion The expression of TrkA is increased short period after the incomplete optic nerve injury and local applying of NGF carried by fibrin sealant does not affect it.

  • [1]Li Y,Holtzman DM,Kromer LF,et al.Regulation of TrkA and ChAT expression in developing rat basal forebrain:evidence that both exogenous and endogenous NGF regulate differentiation of cholinergic neurons[J].J Neurosci,1995,15(4):2888-2905.
  • [2]Henderson CE,Camu W,Mettling C,et al.Neurotrophins promote motor neuron survival and are present in embryonic limb bud[J].Nature,1993,363(6426):266-270.
  • [3]宋连芳,孟凡堂,刘希余,等.实验性脑挫伤后Fos蛋白和NGFR变化与损伤时间关系的研究[J].法医学杂志,2001,17(3):132-136.
  • [4]Zheng WH,Kar S,Quirion R.et al.FKHRL1 andits homologarenew targets of nerve growth factor Trk receptor rsignaing[J].J Neurochem,2002,80(6):1049-1061.
  • [5]Cooper JD,Lindholm D,Sofroniew MV.Reduced transport of[125I]nerve growth factor by cholinergic neurons and down-regulated TrkA expression in the medial septum of aged rats[J].Neuroscience,1994,62(3):625-629.
  • [6]王永刚,张勇,张骐,等.大鼠脊髓中NGF受体TrkA的表达与分布[J].郑州大学学报(医学版),2004,39(3):421-422.
  • [7]董研,刘洪臣,武胜昔,等.TrkA及PPTA mRNA在咬合创伤犬三叉神经节内的表达[J].口腔颌面修复学杂志,2003,4(1):4-8.
  • [8]Yan Q,Johnson EM Jr.An immunohistochemical study of the nerve growth factor receptor in developing rats[J].J Neurosci,1988,8(9):3481-3498.
  • [9]Greenough W,Juraska J.Developmental Neuropsychobiology[M].New York:Academic Press,1986:95-102.
  • [10]Carmingnoto G,Connell MC,Candeo P,et al.Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina[J].Exp Nenrol,1991,111(3):302-311.
  • [11]Loy R,Taglialatela G,Angelucci L,et al.Regional CNS uptake of blood-borne nerve growth factor[J].J Neurosci Res,1994,39(3):339-346.
  • [12]Saika T,Senba E,Noguchi K,et al.Effects of nerve crush and transection on mRNA levels for nerve growth factor receptor in the rat facial motoneurons[J].Brain Res Mol Brain Res,1991,9(1-2):157-160.
  • [13]Cui Q,Tang LS,Hu B,et al.Expression of trkA,trkB,and trkC in injured and regenerating retinal ganglion cells of adult rats[J].Invest Ophthalmol Vis Sci,2002,43(6):1954-1964.
  • [14]袁洪峰,刘少章,贺翔鸽,等.大鼠视神经损伤视网膜节细胞凋亡的实验研究[J].中国临床康复杂志,2002,6(13):1915-1916.
  • [15]赵莉,秦建强,尹方明,等.坐骨神经外植块对脊神经节的感觉神经元高亲合力神经生长因子受体表达的影响[J].神经解剖学杂志,1998,14(3):247-250.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn