Expression of TrKA in animal models with incompletely optic nerve injury and effect of local delivery of NGF on its expression
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2010.03.004
- Key Word:
- TrkA;神经生长因子;视神经损伤;TrkA;Nerve growth factor;Optic nerve injury
Abstract： Objective To explore the expression of high-affinity NGF receptor TrkA and the effect of local delivery of NGF carried by fibrin sealant on its expression in animal models with incompletely optic nerve injury.Methods Male SD rats were randomized into injured group,NGF-treated group and normal control group.The injured models were induced by aneurysm clamp.RT-PCR was employed to detect the mRNA expression of TrkA in the 3 groups on the 3~(rd),5~(th),7~(th),10~(th) d of injury,respectively.Results The relative value in the normal control group was 0.44±0.06.The value of injured and NGF-treated groups was remarkably increased on the 3~(rd) d of injury(P<0.05).The value in the NGF-treated group was increased by 7.1,35.0,19.6 and 9.5 times of that in the control group on the 3~(rd),5~(th),7~（th） and 10~（th） d of injury,respectively;that in the injured group was 8.0,32.4,20.4 and 8.5 times,respectively;no significant difference of the relative value was noted between the 2 groups at different time points(P>0.05).Conclusion The expression of TrkA is increased short period after the incomplete optic nerve injury and local applying of NGF carried by fibrin sealant does not affect it.
- [1]王永刚,张勇,张骐,等.大鼠脊髓中神经生长因子受体TrkA的表达与分布[J].2004,(3).
- [2]董研,刘洪臣,武胜昔,等.TrkA及PPTA mRNA在咬合创伤犬三叉神经节内的表达变化[J].2003,(1).
- [3]袁洪峰,刘少章,贺翔鸽,等.大鼠视神经损伤视网膜节细胞凋亡的实验研究[J].2002,(13).
- [4]宋连芳,孟凡堂,刘希余,等.实验性脑挫伤后 Fos蛋白和 NGFR变化与损伤时间关系的研究[J].2001,(3).
- [5]赵莉,秦建强,尹方明,等.坐骨神经外植块对脊神经节的感觉神经元高亲和力神经生长因子受体表达的影响[J].1998,(3).
- [6]G. Carmignoto,M.C. Comelli,P. Candeo,etc.Expression of NGF receptor and NGF receptor mRNA in the developing and adult rat retina[J].1991,111(3).
- [7]Q, Yan,E M, Johnson.An immunohistochemical study of the nerve growth factor receptor in developing rats.[J].1988,8(9).
- [8]Kar Satyabrata,Quirion Remi,Zheng WenHua.FKHRL1 and its homologs are new targets of nerve growth factor Trk receptor signaling.[J].2002,80(6).
- [9]J.D. Cooper,D. Lindholm,M.V. Sofroniew.Reduced transport of [125I]nerve growth factor by cholinergic neurons and down-regulated trka expression in the medial septum of aged rats[J].1994,62(3).
- [10]C E, Henderson,W, Camu,C, Mettling,etc.Neurotrophins promote motor neuron survival and are present in embryonic limb bud.[J].1993,363(6426).
- [11]Clary DO,Holtzman DM,Knusel B,etc.Regulation of TrkA and ChAT expression in developing rat basal forebrain: evidence that both exogenous and endogenous NGF regulate differentiation of cholinergic neurons.[J].1995,15(4).
- [12]T, Saika,E, Senba,K, Noguchi,etc.Effects of nerve crush and transection on mRNA levels for nerve growth factor receptor in the rat facial motoneurons.[J].1991,9.
- [13]R. Loy,G. Taglialatela,L. Angelucci,etc.Regional CNS uptake of blood-borne nerve growth factor[J].1994,39(3).