Cyclosporin A affects the level of anti-myelin basic protein in serum and myelinoclasis after rat brain trauma

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Author:
CHU Jian-cheng(Department of Neurosurgery,First Affiliated Hospital,Ji'nan University,Guangzhou 510630,China)
LI Wei(Department of Neurosurgery,First Affiliated Hospital,Ji'nan University,Guangzhou 510630,China)
LIU Ling-hui(Department of Neurosurgery,First Affiliated Hospital,Ji'nan University,Guangzhou 510630,China)
LI Hong()
CHENG Shan-cheng(Department of Neurosurgery,First Affiliated Hospital,Ji'nan University,Guangzhou 510630,China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 9, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1671-8925.2010.03.003
Key Word:
Cyclosporin A;Myelin basic protein;Myelinoclasis

Abstract: Objective To investigate the effect of cyclosporin A(CsA)on the level of anti-myelin basic protein(anti-MBP)in the serum and myelinoclasis in rat models after brain trauma.Methods Seventy-seven SD rats were randomized into blank control group(n=7),model group(n=35)and CsA-treated group(n=35).The 0.9% normal saline(5 mg/kg/d)was intraperitoneally injected into the model group and 5 mg/mL CsA(5 mg/kg/d)was injected into the CsA-treated group.Different time points(1,4,10,20 and 30 d)after the brain trauma,the MBP content and anti-MBP titer in the serum were measured using enzyme-linked immunosorbent assay(ELISA)and the degree of myelinoclasis in the brain stem slices was assessed with Marchi's method.Results As compared with that in the blank control group,the MBP and Anti-MBP contents in the model group were significantly increased(P<0.05):anti-MBP in the serum and the degree of myelinoclasis in the brain stem were all significantly increased in the 4th d,both reaching their highest levels on 10~(th) d.No significant differences of the MBP content in the serum between the CsA-treated group and the model group were found the same time points after the injury(P>0.05).However,lower level of anti-MBP in the serum 1 d after the injury and degree of myelinoclasis in the brain stem 4 daRer the injury in the CsA-treated group were observed as compared with those in the model group(P<0.05).Statistical analysis showed an intimate positive correlation between anti-MBP titer in serum and the degree of rnyelinoclasis(r=0.959,P=0.000).Conclusion CsA can affect the immune system to decrease the degree of myelinoclasis in the brain stem through decreasing the level of anti-MBP in the serum of rat models after brain trauma.

  • [1]王双坤,刘佰运,等.创伤性脑损伤的动物实验模型[J].中国交通医学杂志,2005,19(4):327-329.
  • [2]Leker RR,shohami E.Cerebral ischemia and trauma-diffferent etiologies yet similar mechanisms:neuroprotective oppprtanities[J].J Brain Res Rev,2002,39(1):55-73.
  • [3]Xue M,Del Bigio MR.Immune pro-activation exacerbates hemorrhagic brain injury in immature mouse brain[J].J Neuroimmunol,2005,165(1-2):75-82.
  • [4]陈兵,龙霄翱,王东军,等.颅脑损伤患者早期血清IL-6和NSE的变化及临床意义[J].中华神经医学杂志,2005,4(5):477-478.
  • [5]王智坚,黄东健,钱东翔,等.早期肠内营养对重症脑损伤后免疫功能的影响[J].实用医学杂志,2005,21(12):1281-1282.
  • [6]王洪生,赵佩林,孙晓峰,等.大鼠急性局灶性脑挫裂伤后血脑屏障的改变研究[J].中国现代医学杂志,2007,17(2):5-8.
  • [7]文立,李善泉.脑损伤生物学指标研究进展[J].国际神经病学神经外科学杂志,2006,33(6):577-582.
  • [8]Akiyama K,Ichinose S,Omori A,et al.Study of expression of myelin basic proteins(MBPs)in developing rat brain using a novel antibody reacting with four major isoforms of MBP[J].J Neurosci Res,2002,168(1):19-28.
  • [9]李卫.陈善成,汪志刚,等Anti-MBP与大鼠脑组织损伤后脑干继发性脱髓鞘病变的关系[J].南方医科大学学报,2008,28(6):1028-1030.
  • [10]Buki A,Okonkwo Do,PO-vlishock JT.Postinjury cyclosporin A administration limits axonal damage and disconnection in traumatic brain injury[J].J Neurotrauma.1999,16(6):511-521.
  • [11]Kaminska B,Gaweda-Walerych K,Zawadzka M.Molecular mechanisms of neuroprotective action of immunosuppressunts-facts and hypotheses[J].J Cell Mol Med,2004,8(1):45-58.
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