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Curcumin protects dopaminergic cells by inducing heat shock protein 70

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
2
DOI:
10.3760/cma.j.issn.1671-8925.2010.02.003
Key Word:
姜黄素;热休克蛋白70;α-突触核蛋白;蛋白酶体活性;Curcumin;Heat shock protein 70;α-synuclein;Activity ofproteasome

Abstract: Objective To explore the protective effects of curcumin on dopaminergic cells by inducing the expression of heat shock protein 70 (Hsp70), inhibiting anormal expression and aggregation of α-synuclein and promoting proteasome system to degrade abnormal α-synuclein. Methods The cellular model of Parkinson's disease was induced by rotenone in the rat pheochromocytoma strain (PC12 cells) and curcumin was also employed to intervene the cell expression. Cell viability was assessed by MTT assay and the activity of the 3 hydrolases in proteasome was measured by the method of fluorescent anzyme immunoassay. Western blotting was employed to detect the expressions of Hsp70 and α-synuclein and immunofluorescence was used to observe the expression of Hsp70 and the aggregation of α-synuclein. Results The cell viability and the activity of proteasome in the rotenone group were decreased dramatically; the expression of Hsp70 was slightly increased while the expression and aggregation of α-synuclein was increased significantly as compared with those in other groups (P<0.05). Pretreatmant with 0.5 μmol/L and 1.0 μmol/L curcumin resulted in significantly increased cell viability and activity ofproteasome in PC12 cells after co-incubation with 0.1 μmol/L rotenone for 24 h compared with the rotenone group; the expression of Hsp70 was increased significantly and significantly decreased expression and aggregation of α-synuclein was found (P<0.05). The protective effects of 5.0 μmol/L and 10 μmol/L curcumin against the rotenone-induced injury in PC12 cells decreased apparently; when the curcumin reached 10 μmol/L, the activity of chymotrypsin in proteasome statistically lower than that in the rotenone group; the activity of the other 2 enzymes in proteasome were not significantly different from that in the rotenone group (P>0.05). Conclusion Low concentration of curcumin can induce higher expression of Hsp70 and higher activity of proteasome to inhibit the anormal expression and aggregation of α-synuclein in PC 12 cells, thus it can alleviate the rotenone-induced injury in PC 12 cells.

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