Alteration of ATP-sensitive K~+ channel expression in hippocampal neurons after severe chronic hypoxia

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Author:
HUANG Lian-yan(Department of Occupational Health and Occupational medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangdong 510515,China)
LI Bo-xing()
HUANG Xiao-zhou()
SHEN A-dan(Department of Occupational Health and Occupational medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangdong 510515,China)
ZOU Fei(Department of Occupational Health and Occupational medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangdong 510515,China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 9, Issue 01, 2010
DOI:
10.3760/cma.j.issn.1671-8925.2010.01.002
Key Word:
ATP-sensitive K~+ channel;Severe hypoxia;Hippocampal neurons

Abstract: Objective To explore the alteration of ATP-sensitive K~+(K_(ATP))channel expression in hippocampal neurons after severe chronic hypoxia. Methods The hippocampal neurons from 1-week-old rat were incubated and divided into normal control(incubated under 5%CO_2 and 95%air for 8h),hypoxia(incubated under 5%CO2 and 95%N_2 for 8h),hypoxia combined with diazoxide-treated (incubated with 100 μmol/L diazoxide under 5%CO_2 and 95%N_2 for 8h)and hypoxia combined with tolbutamide-treated groups(incubated with 100 μmol/L tolbutamide under 5%CO_2 and 95%N_2 for 8h).Cell apopmsis was identified by MTT.And the mRNA and protein expressions of K_(ATP) channel were estimated by RT-PCR and Western blot analysis.respectively. Results Hypoxia combined with diazoxide-treated group showed a significantly decreased apoptosis rate of neuron as compare with the normal control group 8h after hypoxia(P<0.05);while hypoxia combined with tolbutamide-treated group showed a significantly increased apoptosis rate of neuron compared with the normal control group(P<0.05).The expression of SUR1 in the three hypoxia groups significantly increased as compared with that in the normal control group(P<0.05);however,the expression of Kir6.2 in the three hypoxia groups did not change as compared with that in the normal control group(P>0.05).Conclusion K(ATP),channels can protect the hippocampal neurons under severe chronic hypoxia through the activation of KATP channels and upregulation of expression of KATP channels SUP1 subunit.

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