Effect of Tenuigenin on Mash1 expression and directional differentiation of neural stem cells from mouse hippocampus in vitro

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Author:
ZHU Xiao-feng(Institute of Gerontology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China)
QI Xun-zhong()
SHI Jing(Institute of Gerontology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China)
ZHA Jin-cheng()
TIAN Jin-zhou(Institute of Gerontology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 8, Issue 12, 2009
DOI:
10.3760/cma.j.issn.1671-8925.2009.12.002
Key Word:
Neural stem cell; Cholinergic neuron; Tenuigenin; Mashl

Abstract: Objective To determine the effect of Tenuigenin (TEN) on the Mashl expression in neural stem cells (NSCs) from mouse hippocampus and directional differentiation of its NSCs in vitro. Methods NSCs were generated from the mouse hippocampus within 24 hours after birth and cultured with EGF and bFGF (20 ng/mL, each) in a 50-mm uncoated culture flask. The third-passage NSCs were cultured with TEN medium and divided them into vehicle, 5 mg/L, 20 mg/L and 100 mg/L groups according to different TEN concentrations. Immunocytochemical techniques were employed to detect the NSE-positive neurons and ChAT-positive neurons induced by TEN, and the expression of Mash1 in NSCs affected by TEN was measured by RT-PCR techniques. Results The immunocytochemical and RT-PCR results revealed that ChAT-positive neurons and NSE-positive neurons were detected with statistical significance and 20 mg/L group showed a stronger effect than other concentration groups (P< 0.05). The expression of Mash1 in the 20 mg/L group increased as compared with that in the vehicle group (P<0.05). Conclusion TEN can induce NSCs in vitro differentiating into neurons and cholinergic neurons, and increase the expression of Mashl in NSCs.

  • [1]Haas S,Weidner N,Winkler J.Adult stem cell themrapy in stroke[J].Curr Opin Neurol,2005,18(1):59-64.
  • [2]Jiang Y,Zong CZ.Fingerprint of Polygala tenuifoliaby high performance liquid chromatography[J].Acta PharmSin,2006,41(2):179-183.
  • [3]Bayer AJ,Bullock R,Jones RW,et al.Evaluation of the safety and immunogenicity of synthetic abeta42(AN1792) in patients with AO[J].Neurology,2005,640):94.
  • [4]Chen Q,Cao YG,Zhang CH.Effect of tenuigenin on cholinergic decline induced by β-amyloid peptideand ibotenic acid in rat[J].Acta Pharmaceutica Sinica,2002,37(12):913-917.
  • [5]Wang JC,Harris WA.The role of combinational coding by homeodomain and bHLH transcription factors in retinal cell fate specification[J].DevBiol,2005,285(1):101-115.
  • [6]Wilson SW,Rubenstein JL.Induction and dorsoventral patterni of the telencephalon[J].Neuron,2000,28(3):641-651.
  • [7]Hatakeyama J,Bessho Y,Katoh K,et al.Hes genes regulate size,shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation[J].Development,2004,131(22):5539-5550.
  • [8]Hartung A,Bitton-Worms K,Rechtman MM,et al.Different routes of bone morpho genic protein(BMP) receptor endocytosis influence bmp signaling[J].Mol Cell BioL 2006,26(20):7791-7805.
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