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Tumor necrosis factor-related apoptosis-inducing ligand induces glioma U87 cell apoptosis through the mitochondrial apoptotic pathway

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
9
DOI:
10.3760/cma.j.issn.1671-8925.2008.09.004
Key Word:
肿瘤坏死因子相关凋亡诱导配体;神经胶质瘤;细胞凋亡;线粒体;Tumor necrosis factor-related apoptosis-inducing ligand;Glioma;Apoptosis;Mitochondria

Abstract: Objective To study the mechanism of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-induced apoptosis of glioma U87 cells.Methods Human glioma U87 cells were treated with human recombinant soluble TRAIL(rsTRAIL),and the cell apoptosis was detecmd with flow cytometry with AnnexinV-FITC/PI double staining.Flow cytometry with DiOC6 staining was used to assess the changes in mitochondrial transmembrane potential(△ψm).The relative activity of caspase-3,-8 and-9 Was measmed by colorimetric assay,and the concentration of cytoplasmic cytochrome C(cyt C) determined using enzyme-linked immunosorbem assay.The effects ofcaspase-8 inhibitor(Z-IETD-fmk)on rsTRAIL-induced apoptosis,△ψm,caspase-3,-8 and-9 activities and cyt C concentration were observed. Results RsTRAIL tinle-dependently induced apoptosis and progressive collapse of △ψm in glioma U87 cells,resulting also in caspase-3,-8 and-9activation and elevated cytC concentration.Caspase-8 inhibitor partially antagonized these biological effects induced by rsTRAIL in U87 cells.Conclusion TRAIL initiates a cascade of mitochondrial events by activating caspase-8 and induces apoptosis of glioma U87 cells.

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