Expressions of MMP-9 and TIMP-1 after focal cerebral ischemia-reperfusion in rats

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Author:
CHEN He-cheng(Department of Neurology, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 73005O, China)
YANG Jin-sheng(Department of Neurology, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 73005O, China)
DING Xiao-han()
Sill Xiang-qun(Department of Neurology, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 73005O, China)
YANG Yin-sheng(Department of Neurology, Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou 73005O, China)
Journal Title:
CHINESE JOURNAL OF NEUROMEDICINE
Issue:
Volume 7, Issue 07, 2008
DOI:
Key Word:
Cerebral iscbemia-reperfusion;MMP-9;TIMP-1

Abstract´╝Ü Objective To explore the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) after focal cerebral ischemia-repeffusion injury in rats. Methods Totally 40 Wistar rats were randomly divided into 2 groups: sham-operated group and iscbemia-reperfusion group. A middle cerebral artery occlusion model was constructed in the 25 rats with Longa's method. The expressions of MMP-9 and TIMP-1 were investigated with immunohistochemistry and HE staining at 6, 24, 48, 72 h and 7 d of reperfusion following 2 h ischemia. Results MMP-9 began to be expressed at 6 h reperfusion, and was obviously increased at 24 h and reached the peak level at 48 h, and then, the expression of MMP-9 began to decrease at 72 h to a low level at 7 d. TIMP-1 positive cells began to arise at 6 h reperfusion, peaked at 24 h, decreased at 48 h and remained a low level at 7 d. The expressions of MMP-9 and TIMP-1 were mainly located in vascular endothelial cells, neurons and gitter cells. The expressions of MMP-9 and TIMP-1 were negative in sham-operated group. Conclusion The expressions of MMP-9 and TIMP-1 are induced to increase by focal cerebral ischemia-reperfusion, and vascular endothelial injury may be the main cause to the high expressions of MMP-9 and TLMP-1.

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