You Position: Home > Paper
Determination of MMP-2 and F-actin in glioma cells and investigation of their relationship with the invasion
( views:273, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1671-8925.2008.02.002
- Key Word:
- 神经胶质瘤;基质金属蛋白-2;丝状肌动蛋白;侵袭性
Abstract： 目的 分析胶质瘤细胞分泌基质金属蛋白-2(MMP-2)水平和丝状肌动蛋白(F-actin)的含量,探讨胶质瘤细胞迁移与溶解基质的能力及其与侵袭性的关系.方法 分别用流式细胞术、免疫细胞化学法和transwell体外侵袭实验检测胶质瘤细胞和星形胶质细胞F-actin的含量、MMP-2蛋白的表达水平及侵袭力的强弱,并对两种细胞进行比较分析.结果 胶质瘤细胞F-actin的含量(202.54±11.06)明显高于星形胶质细胞F-actin的含量(62.64±10.23),两者比较有显著性差异(t=-26.258,P=-0.000);胶质瘤细胞MMP-2的表达呈阳性或强阳性,星形胶质细胞MMP-2的表达呈阴性或弱阳性,两组MMP-2阳性表达率分别为96.11%及15.05%,比较有显著性差异(P=-0.000);胶质瘤细胞的侵袭力(246.97±54.48)明显强于星形胶质细胞的侵袭力(39.77±11.46),两组比较有显著性差异(t=20.376,P=0.000).结论 胶质瘤细胞含有大量的F-actin及分泌大量的MMP-2蛋白,高F-actin含量与大量MMP-2蛋白分泌与肿瘤细胞强侵袭力有关.
- [1]杨静,任大宏,郭滟,等.层粘连蛋白及基质金属蛋白酶-2在人脑胶质瘤侵袭过程中的相关研究[J].2005,(2).
- [2]李浩,陈兢,贺民,等.CD147、MMP-2在人脑胶质瘤中的表达及其与预后的关系[J].2007,(3).
- [3]章翔,吴景文,郭衍,等.整合素β1亚基表达与人脑胶质瘤侵袭与复发的关系[J].2003,(7).
- [4]Shi SS,Qin LP,Zhao JX,etc.Gelatinolytic activity of matrix metalloproteinase-2 and matrix metalloproteinase-9 in rat brain after implantation of 9L rat glioma cells.[J].2007,14(5).
- [5]Eline, Menu,Filip, Braet,Maarten, Timmers,etc.The F-actin content of multiple myeloma cells as a measure of their migration.[J].2002,973.
- [6]Tran NL,Symons M,Berens ME,etc.Cell migration and invasion assays.[J].2005,37(2).
- [7]S L, Maidment.The cytoskeleton and brain tumour cell migration.[J].1900,17(6B).