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Investigation on clonal expansion of T-cell receptor Vβ subfamily T cells and cytotoxicity from adult peripheral blood T cells by in vitro induction of U251 cell lysates
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-8925.2008.01.005
- Key Word:
- 神经胶质瘤;T细胞;T细胞受体;克隆性;细胞毒活性
Abstract： 目的 了解神经胶质瘤U251细胞溶解物lysate体外诱导人外周血T细胞的特异性细胞毒作用和T细胞受体(TCR)VB谱系表达和克隆性变化情况. 方法 采用混合淋巴细胞/肿瘤细胞培养方法,以lysate作为刺激原体外诱导外周血单个核细胞增殖.应用LDH释放法检测不同效靶比时诱导后T细胞杀伤U251和Raji细胞的细胞毒活性,同时利用RT-PCR基因扫描技术分析诱导前、后TCR Vβ亚家族的克隆性增殖特点. 结果 基因扫描显示lysate诱导前5例健康人全部TCR Vβ谱型呈高斯(钟型)分布,T细胞均为多克隆;lysate诱导后部分TCR Vβ亚家族T细胞呈单克隆、寡克隆或寡克隆趋势生长.相同效靶比时lysate诱导前、后T细胞对U251细胞杀伤活性之间的差异有统计学意义(P＜0.05);而对Raji细胞的杀伤活性之间的差异无统计学意义(P＞0.05). 结论 U251细胞溶解物lysate在体外可诱导T细胞呈克隆性增殖,此优势增殖的克隆性T细胞对U251细胞具有特异性细胞毒作用.
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