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Up-regulation of the TROY expression after ischemic preconditioning induces tolerance against focal cerebral ischemia in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.10.002
- Key Word:
- 缺血预处理;缺血耐受;TROY
Abstract： 目的 研究缺血预处理(IPC)对局灶性脑缺血的保护作用以及对TNFRSF expressed on the mouse embryo(TROY)表达的影响.方法 33只雄性成年SD大鼠,随机分为预处理6 h组(IPC-6组,11只)、预处理72 h组(IPC-72组,11只)和缺血组(CI组,11只).利用线栓法建立大脑中动脉栓塞(MCAO)致局灶性脑缺血模型.MCAO 10 min作为IPC,IPC-6和IPC-72组分别在IPC后6 h和72 h制作短暂性局灶性脑缺血模型.再灌注24 h后,对所有动物行神经功能缺损评分(NDS),并处死大鼠取脑,应用2%TTC染色测定梗死容积、TUNEL染色研究细胞凋亡情况和免疫组化观察TROY表达变化.结果 与CI组比较,IPC-72组显著改善局灶性脑缺血24 h后大鼠神经功能损害[两组评分分别为2(1.5-3),1(0-2)],减少脑梗死容积[(299.33±70.98)mm3,(69.25±47.66)mm3],抑制细胞凋亡和增强TROY的表达(阳性细胞数分别为42±11,87±17)(P＜0.01).结论 IPC对其后局灶性脑缺血有明显的保护作用,能诱导脑缺血耐受,可能与TROY表达上调相关.
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