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Influence of focal cerebral ischemia on cyclin D1/CDK4 mRNA expressions
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.08.004
- Key Word:
- 脑缺血;细胞周期蛋白D1/CDK4
Abstract： 目的 观察脑缺血后细胞周期蛋白(cyclin)D1和它的酶CDK4基因表达,以及这种表达的改变是否影响神经细胞凋亡.方法 成年雄性SD大鼠32只随机分为假手术组(n=4)和实验组(n=28),实验组再进一步分为7个亚组(再灌注2 h,6 h,12 h,1 d,3 d,7 d和14 d,每组n=4).应用线栓法建立SD大鼠大脑中动脉阻塞/再灌注模型,TUNEL法检测神经细胞凋亡,原位杂交检测cyclin D1和CDK4mRNA的表达. 结果 Cyclin D1 mRNA和CDK4mRNA的表达与凋亡细胞的区域基本相同.再灌注2 h脑组织即开始出现神经细胞凋亡,并于1 d分别在皮层区和纹状体区达高峰(分别为72.80±4.66和87.75±0.85).神经细胞cyclin D1 mRNA和CDK4 mRNA的表达分别于再灌注2 h和6 h开始逐渐增强,并于12 h和1 d达高峰(皮质区分别为94.50±2.75和85.75±3.73,纹状体区分别为88.25±5.06和89.80±2.93).结论 Cyclin D1和CDK4选择性地在形态学完整或已经有改变的缺血侧神经元和少突胶质细胞内表达.Cyclin D1/CDK4mRNA表达可能是诱导细胞凋亡的重要因素之一.
- [1]谢惠芳,徐如祥,魏继鹏,等.大鼠脑缺血再灌注损伤后磷酸化JAK2、STAT3蛋白表达及细胞凋亡[J].2007,(2).
- [2]李方成,陶宗玉,刘安民,等.大鼠脑缺血/再灌注过程中脑型葡萄糖转运体在缺血半影区的表达[J].2005,(5).
- [3]Anna, Abella,Pierre, Dubus,Marcos, Malumbres,etc.Cdk4 promotes adipogenesis through PPARgamma activation.[J].2005,2(4).
- [4]Rose ME,Graham SH,Wu Chen R,etc.Cyclooxygenase-2 activity contributes to neuronal expression of cyclin D1 after anoxia/ischemia in vitro and in vivo.[J].2004,132(1).
- [5]Y, Li,M, Chopp,C, Powers,etc.Immunoreactivity of cyclin D1/cdk4 in neurons and oligodendrocytes after focal cerebral ischemia in rat.[J].1997,17(8).
- [6]Yoh Dobashi,Mitsuhiko Shoji,Takashi Noguchi,etc.A Novel Apoptotic Cascade Mediated by\{CDK4\} in Rat Pheochromocytoma\{PC12\} Cells[J].1999,260(3).
- [7]Datar SA,Galloni M,Edgar BA,etc.Mammalian cyclin D1/Cdk4 complexes induce cell growth in Drosophila.[J].2006,5(6).
- [8]Loeffler JP,Boutillier AL,Kienlen Campard-P.Depolarization regulates cyclin D1 degradation and neuronal apoptosis: a hypothesis about the role of the ubiquitin/proteasome signalling pathway.[J].1999,11(2).
- [9]Berthet C,Klarmann KD,Hilton MB,etc.Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation[J].2006,10(5).
- [10]Morley P,Fournier MC,Monette R,etc.Characterization of cyclin D1 expression in a rat global model of cerebral ischemia.[J].2001,900(1).
- [11]Khrestchatisky M,Tremblay E,Rivera S,etc.Increased cyclin D1 in vulnerable neurons in the hippocampus after ischaemia and epilepsy: a modulator of in vivo programmed cell death?[J].1999,11(1).