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Influence of iNOS-induced apoptosis by mild hypothermia in the ischemic penumbra following bfocal cerebral ischemia-reperfusion in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.08.003
- Key Word:
- 亚低温;脑缺血;诱导型一氧化氮合酶;凋亡;缺血半暗带
Abstract： 目的 通过研究亚低温对大鼠局灶性脑缺血再灌注后诱导型一氧化氮合酶(iNOS)表达和细胞凋亡的影响,探讨亚低温脑保护的可能机制.方法 雄性SD大鼠54只随机分为假手术组、常温缺血组和亚低温组.线栓法制备大脑中动脉闭塞再灌注模型,于缺血后48 h取脑组织,邻片行HE染色,检测各组不同脑区iNOS蛋白表达和细胞凋亡情况,免疫双重染色研究iNOS蛋白表达与细胞凋亡间的关系,同时行NO含量测定.结果 常温缺血组皮质缺血半暗带(IP)区iNOS免疫反应较强,TUNEL阳性细胞也主要位于皮质IP区,免疫双重染色发现TUNEL阳性细胞中存在着iNOS蛋白表达.亚低温组IP区iNOS蛋白表达明显下调,NO产生减少,IP区细胞凋亡的数目也减少.结论 亚低温可能通过抑制IP区iNOS蛋白表达,减少NO产生,阻遏细胞凋亡,从而起到脑保护作用.
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