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Effect of tumor necrosis factor-α on proliferation of in vitro cultured rat VSMC and expression of syndecan-4 protein
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.07.003
- Key Word:
- 肿瘤坏死因子;Syndecan-4;平滑肌细胞;细胞增殖
Abstract： 目的 观察肿瘤坏死因子α(TNF-α)对大鼠胸主动脉血管平滑肌细胞(VSMC)增殖和syndecan-4蛋白表达的影响.方法 体外培养大鼠胸主动脉VSMC,1、10、20、100 ng/mL的TNF-α分别作用并设立对照组(不采取干预措施)进行比较,采用MTS/PES法确定VSMC的增殖状态,利用Western blot蛋白免疫印迹法测定syndecan-4蛋白表达.结果 各组细胞增殖率分别为对照组1.001±0.064,TNF-α1 ng/mL组1.082±0.272,10 ng/mL组1.125±0.198,20 ng/mL组1.566+0.060,100ng/mL组1.215+0.231.统计分析显示低至1 ng/mL的TNF-α仍能明显刺激大鼠VSMC的增殖(与对照组比较,P＜0.05),其中以TNF-α 20 ng/mL组细胞增殖最显著.TNF-α对VSMC的syndecan-4蛋白的表达有显著的增强作用(与对照组比较,P＜0.05),其中也以20ng/mL组增强作用最显著.结论 TNF-α对体外培养大鼠VSMC增殖和svndecan-4蛋白表达均有显著的促进作用.
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