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The relationship between protein aggregation and delayed neuronal death in the cortex after ischemia-reperfusion
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.06.001
- Key Word:
- 皮层神经元;延迟性神经元死亡;蛋白聚集
Abstract： 目的 探讨缺血再灌注后蛋白聚集与大鼠皮层神经元延迟性死亡的关系. 方法 采用20 min全脑缺血的大鼠模型.按照再灌注时间分为5组:假手术组,0.5 h恢复组.4 h恢复组,24 h恢复组,72 h恢复组.采用HE染色光镜下观察缺血再灌注后皮层神经元的延迟性死亡.应用透射电子显微镜观察细胞胞浆内蛋白聚集物.Western blot分析泛素蛋白标记的蛋白聚集物在神经元内的含量变化. 结果 HE染色显示缺血再灌注72h后,可见部分皮层神经元死亡.透射电镜显示再灌注4h后神经元的胞浆内形成了蛋白聚集物.Western blot分析显示,与假手术组相比较,再灌注后0.5 h被泛素标记的蛋白聚集物便开始显著增加,再灌注后4 h和24 h达到峰值,再灌注后72 h含量开始减少,但仍然高于假手术组. 结论 蛋白聚集是导致缺血再灌注后神经元延迟性死亡的一个重要因素.
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