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Improvement of focal cerebral ischemia-reperfusion injury model with intraluminal suture method in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.04.005
- Key Word:
- 缺血再灌注;动物模型;大鼠
Abstract： 目的 研究一种稳定、简便的大鼠局灶性脑缺血再灌注损伤模型制作方法.方法 采用健康SD雄性大鼠,线栓法制作局灶性脑缺血再灌注损伤模型,结扎颈总动脉(CCA)及颈外动脉(ECA),不结扎翼腭动脉,用5-0头皮针于CCA结扎的远端刺一小口插入栓塞线造成缺血,拔出线栓形成再灌注.通过神经功能缺损评分、红四氮唑(TTC)染色和病理学检查等方法评价该模型的可靠性.结果 动物麻醉后一般只需15 min左右即可完成手术,术后大鼠神经功能缺损明显,TTC染色示脑梗塞区苍白,病理学检查显示典型病理表现.结论 该方法缺血效果明确可靠,术式简便,手术时间短,不需具备显微手术操作技巧,是一种理想的制作局灶性脑缺血再灌注损伤模型的方法.
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