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Differentiation of neonatal mouse telencephalon-derived neural stem cells into cholinergic neurons
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.03.005
- Key Word:
- 端脑;神经干细胞;胆碱能神经元;碱性成纤维细胞生长因子
Abstract： 目的 探讨新生小鼠端脑组织神经干细胞是否能够分化成胆碱能神经元.方法 取新生小鼠端脑组织,用无血清方法分离培养神经干细胞;用克隆培养的方法检验培养细胞的干细胞特性;用免疫荧光细胞化学的方法检测神经干细胞标志巢蛋白(nestin)及干细胞诱导分化后神经元标志微管相关蛋白2(MAP2)、星形胶质细胞标志胶质纤维酸性蛋白(GFAP)、胆碱能标志胆碱乙酰转移酶(ChAT);比较不同的诱导分化条件(5%胎牛血清、5%胎牛血清+碱性成纤维细胞生长因子)对胆碱能神经元分化的影响.结果 从新生小鼠端脑组织分离培养出具有自我更新、扩增能力的神经球;各培养基中神经球均为nestin阳性,诱导分化后均能够产生MAP2阳性神经元、GFAP阳性星形胶质细胞以及ChAT阳性的胆碱能神经元.分化培养中加入碱性成纤维细胞生长因子能够提高胆碱能神经元分化的比例.结论 新生小鼠端脑组织神经干细胞能够分化成胆碱能神经元.
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