You Position: Home > Paper
Study on the related biological characteristics of human bone marrow stromal cells as donor cells during nuclear transfer
( views:162, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.03.002
- Key Word:
- 骨髓基质细胞;供体细胞;细胞骨架;染色体;细胞周期;端粒酶
Abstract： 目的 研究作为核移植供体细胞的人骨髓基质细胞相关生物学特性.方法 应用密度梯度离心法分离培养人骨髓基质细胞,并对其进行形态观察、免疫荧光分析细胞骨架结构、端粒酶活性测定、核型及细胞周期分析.结果 培养的细胞具有正常的大小、形态、细胞骨架结构;在融合密度达80%～90%时,G0+G1期细胞所占的比例较高,约占92.58%;检测传至第7代的人骨髓基质细胞具有正常染色体数目(46,XY),端粒酶活性为0.567.结论 形态良好、染色体数目正常的人骨髓基质细胞可以作为核移植的供体细胞.
- [1]滕晓华,徐如祥,邹雨汐,等.不同浓度血清对大鼠骨髓基质细胞生长的影响[J].2005,(7).
- [2]陆凤花,石德顺,韦英明,等.不同融合条件对水牛体细胞核移植效果的影响[J].2005,(1).
- [3]周虎田,徐如祥,姜晓丹,等.胚脑来源与骨髓来源神经干细胞的比较：应用原子力显微镜的观察[J].2005,(5).
- [4]杨素芳,石德顺,韩杰,等.供体核种类对水牛核移植效果的影响[J].2004,(3).
- [5]韩伟,章孝荣.哺乳动物体细胞核移植中供体细胞的研究进展[J].2006,(5).
- [6]R S, Prather,M M, Sims,N L, First.Nuclear transplantation in early pig embryos.[J].1989,41.
- [7]Pittenger, Mark F.,Mackay, Alastair M.,Beck, Stephen C.,etc.Multilineage Potential of Adult Human Mesenchymal Stem Cells.[J].1999,284(5411).
- [8]H, Funahashi,T C, Cantley,T T, Stumpf,etc.In vitro development of in vitro-matured porcine oocytes following chemical activation or in vitro fertilization.[J].1994,50.
- [9]A F, Tsyb,A G, Konopliannikov,A I, Kolesnikova,etc.[Production of cell cultures from mesenchymal stem cells of the human bone marrow and their use in medicine].[J].2004,(9).