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Experimental study on the combined treatment for rat C6 gliomas with EGFR antisense gene therapy and gamma knife radiosurgery
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-8925.2007.01.005
- Key Word:
- 神经胶质瘤;γ-刀;放射外科;反义EGFR基因治疗
Abstract： 目的 研究表皮生长因子受体(EGFR)反义RNA联合γ-刀治疗对鼠C6胶质瘤的生长抑制作用. 方法 将1×106/15 mLC6胶质瘤细胞(每只15 mL)接种至SD大鼠右侧尾状核头部,建立SD大鼠C6脑胶质瘤模型.6 d后进行MRI检查,确定成瘤后随机分为4组(A:对照组;B:EGFR反义RNA基因治疗组;C:γ-刀治疗组;D:EGFR反义RNA+γ-刀治疗组),每组12只.B、D组在第6、8天进行脂质体介导的反义EGFR质粒治疗.C、D组第10天进行γ-刀放射外科治疗,给予边缘剂量15 Gy.A组未作任何治疗.第11天每组处死2只大鼠进行生物学特性检测,包括PCNA(免疫组化法)、凋亡(TUNEL法);其余10只在γ-刀治疗后1、2、4及8周进行MRI检查,并观察生存期. 结果 EGFR反义RNA联合γ-刀治疗组较单一治疗组动物生存期显著延长(P＜0.05),肿瘤细胞PCNA阳性率下降、凋亡率增加,MRI检查证实肿瘤生长缓慢. 结论 γ-刀放射外科联合EGFR反义RNA治疗C6胶质瘤大鼠,具有更高的肿瘤生长抑制率及肿瘤细胞凋亡率,动物生存时间显著延长.
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