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Expressions of Ref-1 ,NF-κB and IκB after transient focal cerebral ischemia-reperfusion in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.12.008
- Key Word:
- 脑缺血再灌注;Ref-1蛋白;NF-κB;IκB
Abstract： 目的 研究大鼠局灶性脑缺血再灌注损伤后Ref-1蛋白、NF-κB及IκB的动态变化.方法 采用大脑中动脉线栓法制作局灶性脑缺血再灌注模型,应用免疫组化法检测Ref-1、NF-κB、IκB的动态表达.结果 与对照组及假手术组相比,脑缺血再灌注后,随着再灌注时间的延长,Ref-1蛋白、IκB阳性细胞数逐渐减少(P＜0.05),NF-κB蛋白阳性细胞数逐渐增加(P＜0.05).结论 脑缺血区再灌注损伤可导致Ref-1蛋白酶活性下降、IκB降解,引起NF-κB的激活,进一步导致自由基大量产生,加重了细胞内DNA的损伤,从而促进损伤区的神经元凋亡.
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