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Migration of bone marrow stromal cells in vitro induced by Fractalkine
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.12.006
- Key Word:
- 骨髓基质细胞;趋化因子;迁移;体外
Abstract： 目的 探讨趋化因子Fractalkine对骨髓基质细胞(BMSCs)的体外迁移作用.方法 采用全骨髓法培养成年Wistar大鼠BMSCs,取第五代BMSCs行免疫荧光鉴定;后通过细胞免疫荧光及RT-PCR的方法检测BMSCs表达趋化因子受体CX3CR1的情况,利用Boyden小室法探讨趋化因子Fractalkine对BMSCs的体外趋化作用及其特异性.结果 第五代BMSCs都表达间充质干细胞标记物Vimentin、Laminin及Fibronectin;CX3CR1细胞免疫荧光及RT-PCR结果证实BMSCs表达趋化因子受体CX3CR1,趋化因子Fractalkine(5、50、500ng/mL)体外可趋化BMSCs迁移,抗Fractalkine多克隆抗体可对抗其趋化迁移作用.结论 Fractalkine/CX3CR1通路参与BMSCs体外迁移,为进一步研究BMSCs的迁移机制提供了理论依据.
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