You Position: Home > Paper
Changes of electrophysiological characteristics in striatal neurons of rats with levodopa-induced dyskinesias
( views:421, downloads:28 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.09.003
- Key Word:
- 左旋多巴;异动症;帕金森病;多巴胺受体;细胞外记录
Abstract： 目的 探讨纹状体神经元电生理活动的变化在左旋多巴诱发异动症(LID)发生中的作用及意义.方法 32只大鼠共分为3组:对照组(n=10),帕金森病(PD)组(n=12),LID组(n=10).6-羟基多巴胺(6-OHDA)立体定位注射制备偏侧PD大鼠模型,左旋多巴腹腔注射治疗4周诱发LID大鼠模型.两组模型分别尾静脉注射多巴胺D1、D2受体激动剂SKF-38393、quinpirole,受体拮抗剂SCH-23390、haloperidol,采用微电极细胞外记录技术检测纹状体棘状神经元(SMSNs)电生理活动的变化.结果 LID组SMSNs的自发性电活动较对照组及PD组明显增多(P＜0.05).SKF-38393对LID组SMSNs自发性电活动的抑制作用呈浓度依赖性,LD组SMSNs的半抑制浓度(IC50)较PD组明显下降(P＜0.05).Quinpirole对LID组SMSNs的IC50与PD组相比无显著性差异(P＞0.05).结论 LID大鼠SMSNs的自发性电活动增强,D1受体介导的神经元电活动敏感性增高.
- [1]曹学兵,孙圣刚,王岚,等.左旋多巴诱发异动症大鼠皮质纹状体突触超微结构与功能的变化[J].2004,(2).
- [2]徐岩,孙圣刚,曹学兵.左旋多巴诱发异动症大鼠模型纹状体神经元可塑性的研究[J].2004,(3).
- [3]Venderova K,Lee J,Brotchie JM.Levodopa-induced dyskinesia in Parkinson's disease.[J].2005,112(3).
- [4]Wilson CJ,Kawaguchi Y.The origins of two-state spontaneous membrane potential fluctuations of neostriatal spiny neurons.[J].1996,16(7).
- [5]Soubrouillard C,Blin O,Goetz CG,etc.Induction by dopamine D1 receptor agonist ABT-431 of dyskinesia similar to levodopa in patients with Parkinson disease.[J].2001,58(2).
- [6]Centonze D,Bernardi G,Picconi B,etc.Therapeutic doses of L-dopa reverse hypersensitivity of corticostriatal D2-dopamine receptors and glutamatergic overactivity in experimental parkinsonism.[J].2004,127(Pt.7).
- [7]Christopher Bishop,Dev P. Kamdar,Paul D. Walker.Intrastriatal serotonin 5-HT2 receptors mediate dopamine D1-induced hyperlocomotion in 6-hydroxydopamine-lesioned rats[J].2003,50(2).
- [8]Cenci MA,Schulzer M,Bjorklund A,etc.Embryonic ventral mesencephalic grafts improve levodopa-induced dyskinesia in a rat model of Parkinson's disease.[J].2000,123(Pt.7).