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Relationship of telomerase activity with proliferation and differentiation in neural stem cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.09.001
- Key Word:
- 神经干细胞;端粒酶;增殖;分化
Abstract： 目的 探讨体外培养的神经干细胞端粒酶活性与细胞增殖、分化的关系,以及细胞分化后端粒酶逆转录酶的表达情况.方法 采用无血清培养法从新生大鼠脑皮质分离培养神经干细胞;通过免疫荧光细胞染色鉴定神经干细胞;细胞计数法检测细胞的增殖情况;TRAP-ELISA法测定神经干细胞的端粒酶活性;RT-PCR法和Western-blot法测定细胞分化前后的端粒酶逆转录酶的表达.结果 从新生大鼠脑皮质分离培养的神经干细胞具有端粒酶活性;体外培养12周内,神经干细胞的端粒酶活性未见变化,细胞的增殖速率亦未见明显不同;神经干细胞分化后端粒酶活性丧失,端粒酶逆转录酶的mRNA和蛋白质也均未见表达.结论 在体外培养过程中,大鼠脑神经干细胞的端粒酶活性和细胞增殖速率未见变化;神经干细胞分化后端粒酶活性丧失,可能是由于端粒酶逆转录酶停止表达所致.
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