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Effect of saikosaponins on glutamate cells of hippocampus in PTZ-induced slow-kindling rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.08.002
- Key Word:
- 柴胡总皂甙;戊四氮;癫痫;谷氨酸;SD大鼠
Abstract： 目的 研究柴胡总皂甙对戊四氮(PTZ)慢性点燃癫痫模型大鼠海马区谷氨酸(Glu)阳性细胞表达的影响.方法 48只健康SD大鼠被随机分为6组,即空白组(A组)、生理盐水组(B组)、丙戊酸钠(VPA)组(C组)和柴胡总皂甙高、中、低三种剂量组(D组、E组、F组),每组8只,除A组不做处理外,其他各组采用腹腔注射PTZ慢性点燃造模,造模同时给予VPA、柴胡总皂甙等不同处理因素,连续4周后取脑组织切片进行Glu免疫组化染色,从阳性细胞数、灰度值分析.结果 在CA1区,B组海马阳性细胞数高于A、C、D、E、F组,有显著性差异(P＜0.05),B组海马各区阳性细胞灰度值低于其他各组,与A、C、D各组比较,有显著性差异(P＜0.05);而在CA2区和DG区,B组阳性细胞数、灰度值与各组差异无统计学意义.结论 柴胡总皂甙可以影响PTZ点燃大鼠海马CA1区的Glu表达水平,从而抑制PTZ慢性点燃大鼠的痫性发作.
- [1]林元相,康德智,陈振斌,等.癫痫灶的超微结构观察及与癫痫发生机制的关系探讨[J].2004,(4).
- [2]谢炜,鲍勇,于礼建,等.柴胡总皂甙对戊四氮慢性点燃大鼠痫性发作及脑电图的影响[J].2006,(2).
- [3]谢炜,鲍勇,于礼建,等.柴胡总皂甙对对戊四氮慢性点燃大鼠海马 GFAP表达的影响[J].2006,(4).
- [4]McNamara JO,Bausch SB.Contributions of mossy fiber and CA1 pyramidal cell sprouting to dentate granule cell hyperexcitability in kainic acid-treated hippocampal slice cultures.[J].2004,92(6).
- [5]M J, During,D D, Spencer.Extracellular hippocampal glutamate and spontaneous seizure in the conscious human brain.[J].1993,341(8861).
- [6]Christian, Wozny,Anatol, Kivi,Thomas-Nicolas, Lehmann,etc.Comment on "On the origin of interictal activity in human temporal lobe epilepsy in vitro".[J].2003,301(5632).
- [7]Shao LR,Dudek FE.Increased excitatory synaptic activity and local connectivity of hippocampal CA1 pyramidal cells in rats with kainate-induced epilepsy.[J].2004,92(3).
- [8]Huttmann K,Steinhauser C,Schramm J,etc.Enhanced relative expression of glutamate receptor 1 flip AMPA receptor subunits in hippocampal astrocytes of epilepsy patients with Ammon's horn sclerosis.[J].2004,24(8).
- [9]Ki-Hyung, Kim,Jeoung-Hee, Ha,Seung-Hyuk, Chung,etc.Glutamate and GABA concentrations in the cerebellum of novel ataxic mutant Pogo mice.[J].2003,4.
- [10]M J, During,C W, Symes,P A, Lawlor,etc.An oral vaccine against NMDAR1 with efficacy in experimental stroke and epilepsy.[J].2000,287(5457).