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Expression changes of nitric oxide synthase after cerebral ischemia/reperfusion injury in rats and the effect of timosaponin on it
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.08.001
- Key Word:
- 缺血再灌注损伤;一氧化氮合酶
Abstract： 目的 观察脑缺血再灌注损伤后3型一氧化氮合酶(NOS)在脑内表达的变化,了解知母总皂苷(SAaB)对它们表达的影响.方法 大鼠灌胃给药7 d,采用线栓法制作大鼠局灶性脑缺血再灌注损伤模型,观察大鼠神经功能损害,利用免疫组织化学法观察脑组织中3型NOS表达.结果 缺血再灌注组大鼠表现出明显神经功能障碍,神经元型NOS(nNOS)含量明显升高,内皮型NOS(eNOS)仅轻度升高.SAaB低、高剂量治疗组大鼠神经功能障碍较轻,同时脑组织中nNOS表达明显降低,而eNOS的表达增强,经图像分析比较后显示各组表达强度差异具有统计学意义(P＜0.05).结论 各型NOS的表达变化在脑缺血再灌注损伤的机制中起着重要作用,推测SAaB因其减低nNOS表达同时增加eNOS表达的作用,而表现出一定的神经保护作用.
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