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Inhibitory effects of antisense hTERT cDNA on proliferation of human glioma cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.07.005
- Key Word:
- 人端粒酶催化亚基反义cDNA;神经胶质瘤;端粒酶;基因治疗
Abstract： 目的探讨人端粒酶催化亚基(hTERT)反义cDNA(pAdeasy-hTERT)在体外对胶质瘤细胞生长的抑制作用.方法将腺病毒介导的pAdeasy-hTERT作用于人胶质瘤细胞系U251,用MTT法检测细胞存活率、端粒酶重复序列扩增法测定端粒酶活性、Western杂交鉴定hTERT蛋白的表达、RT-PCR法检测hTERT cDNA水平、PCNA观察肿瘤细胞的凋亡情况以及用流式细胞仪对转染后肿瘤细胞的周期进行分析.结果pAdeasy-hTERT在体外明显抑制肿瘤细胞生长,降低端粒酶活性,抑制hTERT表达.结论pAdeasy-hTERT显著抑制人胶质瘤细胞生长,可成为恶性胶质瘤基因治疗的靶基因.
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