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Stereotaxic transplantation of adipose tissue derived stromal cells into lateral cerebral ventricle for treatment of focal cerebral ischemia in rats

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Author:: No author available

Journal Title:: CHINESE JOURNAL OF NEUROMEDICINE

Issue:: 7

DOI:: 10.3760/cma.j.issn.1671-8925.2006.07.001

Key Word:: 脂肪组织源性基质细胞;立体定向;移植;脑缺血

Abstract：目的探讨脂肪组织源性基质细胞(ADSCs)经侧脑室立体定向移植治疗大鼠局灶性脑缺血的机制.方法体外培养、扩增ADSCs,常规细胞免疫化学染色鉴定其种类;细胞移植前3 d,加入含BrdU的培养液培养3 d.缺血再灌注大鼠模型第4天经侧脑室移植ADSCs,于移植前、移植后第1、7、14、21、28天进行评分.移植后28 d,断头取脑,各组一部分脑片经红四氮唑(TTC)染色法检测脑梗死体积,另一部分切片用原位TUNEL法检测细胞凋亡;用免疫荧光双标法检测脑源性神经营养因子(BDNF)在有BrdU活性的ADSCs的表达.结果大多数ADSCs呈CD44(间质干细胞特征性标志物)阳性,CD114抗体(单核巨嗜细胞标志物)及CD34抗体(造血干细胞标志物)呈阴性.ADSCs组和对照组神经功能损害评分于脑缺血后21 d、28 d呈显著性差异(P＜0.01),脑梗死体积无显著性差异(P＞0.05);免疫荧光双标法检测显示大部分BrdU标记的ADSCs迁移到梗死周边区,ADSCs组细胞凋亡数明显低于对照组(P＜0.01).结论经侧脑室立体定向移植的ADSCs可能是通过减少梗死周边区细胞的凋亡促进大鼠局灶性脑缺血的神经功能恢复.

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