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Neuroprotective effects of Rifampicin on MPTP-induced DA neurons apoptosis in C57BL mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.06.002
- Key Word:
- 利福平;1-甲基-4-苯基 -1,2,3,6- 四氢吡啶(MPTP);帕金森病;凋亡;C57BL小鼠
Abstract： 目的观察利福平(RFP)对1-甲基-4苯基-1,2,3,6-四氢吡啶(MPTP)致帕金森病(PD)C57BL小鼠模型的神经保护作用.方法用MPTP建立C57BL小鼠PD模型,在应用MPTP前或后给予RFP,通过行为学观察、Nissl染色、免疫组织化学染色,观察RFP对PD小鼠模型的行为学表现及黑质致密部(SNc)Nissl、TH、Bcl-2、caspase-3阳性细胞的影响.结果小鼠注射MPTP后出现震颤、竖尾、竖毛、运动迟缓、步态不稳、肢体僵硬,活动明显减少,同时SNc的Nissl、TH阳性细胞明显减少,Bcl-2阳性细胞有所增多,caspase-3阳性细胞明显增多;应用RFP后较MPTP组症状减轻,Nissl、TH、Bcl-2阳性细胞增多,而caspase-3阳性细胞减少;预使用RFP组作用最为明显.结论RFP对MPTP所致的PD小鼠中脑多巴胺能神经元凋亡具有保护作用.
- [1]姚庆和,高国栋.MPTP的毒性机制与帕金森病动物模型[J].2004,(1).
- [2]陈滢,陈晓春.MPTP诱导小鼠黑质神经元凋亡[J].2001,(2).
- [3]Y, Namba,K, Kawatsu,S, Izumi,etc.Neurofibrillary tangles and senile plaques in brain of elderly leprosy patients.[J].1992,340(8825).
- [4]Shoji A..Inhibition of Amyloid beta Protein Aggregation and Neurotoxicity by Rifampicin[J].1996.
- [5]C, Calleja,J M, Pascussi,J C, Mani,etc.The antibiotic rifampicin is a nonsteroidal ligand and activator of the human glucocorticoid receptor.[J].1998,4(1).
- [6]主编梁晓俐.病理学基础与实验技术[M].2004.
- [7]Smirnov AV,Zettl UK,Mix E,etc.Rifampin reduces production of reactive oxygen species of cerebrospinal fluid phagocytes and hippocampal neuronal apoptosis in experimental Streptococcus pneumoniae meningitis.[J].2000,181(6).
- [8]Sander V,Pohl K,Nau R,etc.Rifampin followed by ceftriaxone for experimental meningitis decreases lipoteichoic Acid concentrations in cerebrospinal fluid and reduces neuronal damage in comparison to ceftriaxone alone.[J].2003,47(4).
- [9]Yulug B,Bahr M,Kilic U,etc.Rifampicin attenuates brain damage in focal ischemia.[J].2004,996(1).
- [10]N.A Tatton,S.J Kish.In situ detection of apoptotic nuclei in the substantia nigra compacta of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice using terminal deoxynucleotidyl transferase labelling and acridine orange staining[J].1997,77(4).