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Observation on intracerebrally transplanted BMSCs-D-NSCs modified by TH and EGFP gene in hemiparkinsonism monkey
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.05.004
- Key Word:
- 帕金森病;基因治疗;神经干细胞;脑内移植;酪氨酸羟化酶
Abstract： 目的评价外源性酪氨酸羟化酶(TH)和增强型绿色荧光蛋白(EGFP)基因修饰的骨髓基质细胞源神经干细胞(BMSCs-D-NSCs)在猴帕金森病(PD)模型脑内移植后长期存活情况及其功能评定. 方法应用MPTP制成偏侧PD猴模型.将构建的pEGFP-hTH转染培养的自体BMSCsD-NSCs,而后植入PD猴模型脑内,观察其行为学变化,并进行单光子发射计算机体层摄影术(多巴胺转运蛋白显像)和正电子发射计算机体层摄影术(葡萄糖代谢显像)检查.用免疫组化法检测移植细胞的存活情况. 结果成功构建pEGFP-hTH和pEGFP-C2转染的BMSCs-D-NSCs中TH和EGFP基因均有效表达;细胞移植后5个月PD猴症状仍有所改善.多巴胺转运蛋白显像见细胞移植组术侧纹状体平均标准摄取值低于对侧及健康猴,而高于PD猴术侧.葡萄糖代谢显像见细胞移植组术侧纹状体平均标准摄取值低于对侧及健康猴,而高于PD猴术侧,而细胞移植组间无明显差异.免疫组化发现,细胞移植术后5个月移植区仍存在大量TH、EGFP阳性细胞. 结论 pEGFP-hTH和pEGFP-C2转染的BMSCs-D-NSCs在体外和体内均长期表达外源性TH、EGFP基因,基因工程细胞在PD猴脑内存活并发挥治疗作用.
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