Functional differentiation of MSCs into dopamine-producing cells after engrafting into the striatum of Parkinson's rat
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.04.005
- Key Word:
- 骨髓间充质干细胞;分化;多巴胺能神经元;帕金森病;rMSCs;Differentiation;Dopamine neu-rons;Parkinson' s disease
Abstract： Objective To investigate the factors ofrMSCs differentiation into dopamine-producing cells and its recovery potential of rat Parkinson model in vivo. Methods To better repair the 6-hydroxydopamine (6-OHDA) unilateral lesioned dysfunction in the substantia nigra of rats, rMSCs was prepared from adult Sprague-Dawley (SD) rat. They were firstly proliferated and differentiated in vitro into neuron-like cells in serum-free medium with a Chinese medicine, Musk's polypeptide named Musk-1, then grafted into Parkinson rats that had unilateral Dopaminergic (DA) - depleted striatum. Results 2 h after Musk-1 was added, cells started to differentiate into neuron-like cells, making those cell spheres nestin,NSE, NF-H positive. After cell implantation, animals promoted significant improvement in function(apomorphine-induced rotation ameliorated in 25 grafted animals and persisted for 56 days on average)compared to the lesion-control group (P＜0.001). Histological analysis confirmed a large number of rMSCs-derived cells survived well in the transplantation zoon and migrated around the host lesion as far as 5millimeters, followed by developing into DA neuron phenotype on approximately 50%～55% of overall cells tested by the DA neuron marker tyrosine hydroxylase (TH). Conclusion These findings demonstrated that grafted rMSCs-derived cells can survive, migrate, and spontaneously develop into Dopamine-producing cells in striatum and restore behavioral properties of neurons expected from the midbrain.
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