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Amplification and differentiation towards neuron-like cells of human umbilical cord derived mesenchymal stem cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2006.03.004
- Key Word:
- 脐带;间充质干细胞;体外扩增;诱导分化;神经元样细胞
Abstract： 目的探讨人脐带间充质干细胞(MSCs)的体外分离、纯化、扩增和向神经元样细胞的定向诱导分化,以期为脐带MSCs的神经移植提供理论依据.方法无菌条件下收集剖宫产新生儿脐带,酶消化法获取MSCs,进行培养.用流式细胞仪检测MSCs的表面标志.取扩增3,5,10代的MSCs分别向神经元样细胞诱导,用免疫组化和RT-PCR法检测神经元样细胞特异性标志.结果脐带富含MSCs,且脐带MSCs(UCMSCs)强表达CD13、29、CD44、CD105,弱表达CD106,不表达CD34、CD11a、CD14、CD33、CD45.神经条件培养基诱导后的细胞平均有70%左右呈现典型的神经元样表型.免疫组化法检测发现不同代数的MSCs经诱导后均表达nestin,NSE,NeuN,NF-M,弱表达GFAP.RT-PCR显示诱导后NSEmRNA表达增加.结论MSCs存在于人脐带中,并且在体外有较强的增殖能力,特定条件下能够分化为神经元样细胞.
- [1]郭再玉,姜晓丹,徐如祥,等.骨髓基质细胞源性神经干细胞体外分化及电生理特性的研究[J].2005,(6).
- [2]Gallo G,Kostura L,Howard L,etc.Reevaluation of in vitro differentiation protocols for bone marrow stromal cells: Disruption of actin cytoskeleton induces rapid morphological changes and mimics neuronal phenotype.[J].2004,77(2).
- [3]Francesco P, Jori,Marco A, Napolitano,Mariarosa A B, Melone,etc.Molecular pathways involved in neural in vitro differentiation of marrow stromal stem cells.[J].2005,94(4).
- [4]Massy M,Bron D,Lagneaux L,etc.Bone marrow-derived mesenchymal stem cells already express specific neural proteins before any differentiation.[J].2004,72(7).
- [5]Korkko J,Ibberson D,DiGirolamo C,etc.MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages.[J].2001,19(5).
- [6]Prockop DJ,Woodbury D,Black IB,etc.Adult rat and human bone marrow stromal cells differentiate into neurons.[J].2000,61(4).
- [7]Deliliers GL,Calzarossa C,Rimoldi SG,etc.Neuro-glial differentiation of human bone marrow stem cells in vitro.[J].2005,193(2).
- [8]Lu P,Tuszynski MH,Blesch A.Induction of bone marrow stromal cells to neurons: Differentiation, transdifferentiation, or artifact?[J].2004,77(2).
- [9]Mattson MP,Rao MS.Stem cells and aging: expanding the possibilities.[J].2001,122(7).