You Position: Home > Paper
Recombinant human hepatocyte growth factor in cellular apoptosis of glioma induced by mitomycin C
( views:284, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1671-8925.2005.11.004
- Key Word:
- 神经胶质瘤;肿瘤细胞;人类重组肝细胞生长因子;细胞凋亡
Abstract： 目的探讨人类重组肝细胞生长因子(rhHGF)在丝裂霉素C(MMC)诱导人胶质瘤U251细胞凋亡中的作用.方法采用倒置相差显微镜和透射电镜观察细胞凋亡变化,MTT法检测细胞生长抑制率,TUNEL法和流式细胞术(FCM)研究U251细胞凋亡及细胞周期变化,分析不同浓度的rhHGF对低剂量MMC诱导U251细胞凋亡的影响.结果MMC组U251细胞出现细胞凋亡特有的形态学特征,其抑制率和凋亡指数(AI)分别为(43.7±3.2)%和(63.2±3.4)%,较不同浓度的rhHGF加MMC组和空白对照组显著增高(P＜0.05).随着rhHGF浓度的增加,细胞生长抑制率和凋亡指数呈递减趋势.FCM检测结果表明,MMC主要诱导G0/G1期细胞凋亡而出现凋亡峰,凋亡率25.86%;10和20μg/L rhHGF能特异性抑制MMC诱导U251细胞凋亡,凋亡率分别为15.14%和11.12%. 结论rhHGF能抑制MMC诱导的U251细胞凋亡,可能与胶质瘤的复发、耐药等临床特性有关.
- [1]Culberson JC,Darke PL,Drakas RA,etc.Biochemical and structural characterization of a novel class of inhibitors of the type 1 insulin-like growth factor and insulin receptor kinases[J].2005,44(27).
- [2]Kotaro, Fukushima,Shingo, Miyamoto,Kiyomi, Tsukimori,etc.Tumor necrosis factor and vascular endothelial growth factor induce endothelial integrin repertories, regulating endovascular differentiation and apoptosis in a human extravillous trophoblast cell line.[J].2005,73(1).
- [3]Cancrini A,Scarpino S,Di Napoli A,etc.Hepatocyte growth factor (HGF) downregulates thrombospondin 1 (TSP-1) expression in thyroid papillary carcinoma cells.[J].2005,205(1).
- [4]Kawamata H,Okui N,Aruga S,etc.Biological role of HGF/MET pathway in renal cell carcinoma.[J].1999,161(3).
- [5]Ken-ichiro Kosai,Kunio Matsumoto,Shigekazu Nagata,etc.Abrogation of Fas-Induced Fulminant Hepatic Failure in Mice by Hepatocyte Growth Factor[J].1998,244(3).
- [6]S, Fan,J A, Wang,R Q, Yuan,etc.Scatter factor protects epithelial and carcinoma cells against apoptosis induced by DNA-damaging agents.[J].1998,17(2).