You Position: Home > Paper
Comparative analysis of G-banding in human neural stem cell derived tumor cell lines
( views:286, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2005.03.004
- Key Word:
- 神经干细胞;核型;吉姆萨显带
Abstract： 目的比较人类神经干细胞源肿瘤细胞系和人类神经干细胞系染色体G-显带的差异,探讨神经干细胞成瘤性机制.方法制备人类神经干细胞系和其来源的肿瘤细胞系(T1和T2)细胞分裂中期染色体,胰酶处理后,经吉姆萨染色,用cytoscan-karyotyping FISH＆CGH软件进行G-显带分析.结果人类神经干细胞源肿瘤细胞系染色体发生缺失、易位、双着丝粒等结构改变以及染色体数目改变,核型从46,XX到69,XX不等.结论人类神经干细胞来源的肿瘤细胞系染色体发生异常改变,表现为数量变化和结构重排.
- [1]O, Brüstle,K N, Jones,R D, Learish,etc.Embryonic stem cell-derived glial precursors: a source of myelinating transplants.[J].1999,285(5428).
- [2]Xiaoxia, Li,Jinchong, Xu,Yun, Bai,etc.Isolation and characterization of neural stem cells from human fetal striatum.[J].2005,326(2).
- [3]F, Ciccolini,C N, Svendsen.Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouse striatal precursor cells: identification of neural precursors responding to both EGF and FGF-2.[J].1998,18.
- [4]J, Fricker.Human neural stem cells on trial for Parkinson's disease.[J].1999,5(4).
- [5]Weidner N,Haas S,Winkler J.Adult stem cell therapy in stroke.[J].2005,18(1).
- [6]Buhrle C,Xia Y,Kustermann E,etc.Host-dependent tumorigenesis of embryonic stem cell transplantation in experimental stroke.[J].2003,23(7).
- [7]Kokaia Z,Satrustegui J,Arco A,etc.BDNF gene transfer to the mammalian brain using CNS-derived neural precursors.[J].1999,6(11).
- [8]Broaddus WC,Holloway KL,Fillmore HL,etc.Progress in cerebral transplantation of expanded neuronal stem cells.[J].2004,100(4).
- [9]Levy YS,Melamed E,Offen D,etc.Embryonic and adult stem cells as a source for cell therapy in Parkinson's disease.[J].2004,24(3).
- [10]Ourednik V,Yang W,Aboody KS,etc.From the cover: neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas.[J].2000,97(23).