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Change of hypothalamic G protein-coupled receptor kinase in rats early after severe trauma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1671-8925.2005.02.005
- Key Word:
- G蛋白偶联受体激酶;促肾上腺皮质激素释放激素;下丘脑;创伤和损伤
Abstract： 目的研究G蛋白偶联受体激酶(GRK)在大鼠下丘脑的分布和严重创伤后时相变化及其与应激早期下丘脑促肾上腺皮质激素释放激素(CRH)分泌的关系.方法采用胸部撞击和单侧股骨中段骨折进行致伤.50只成年Wistar大鼠随机分为正常对照组和创伤组,通过免疫组化实验研究GRK在下丘脑的分布,Western blot实验研究室旁核GRK的蛋白表达,RT-PCR技术研究室旁核CRH的转录活性.结果在下丘脑室旁核有GRK2/3的分布,但未发现GRK6的阳性染色;GRK2/3的总蛋白在创伤后30 min显著下降(P＜0.05),90 min恢复近正常水平;CRH mRNA表达在伤后30 min显著升高(P＜0.05),60 min、90 min的升高非常显著(P＜0.01).结论在创伤应激过程早期GRK2/3的蛋白表达受到了抑制,推测下丘脑室旁核促肾上腺皮质激素释放激素1型受体(CRHR1)的同源性脱敏受到了抑制,间接促进了下丘脑室旁核CRH的分泌.
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