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Primary study on intracellular labeling of bone marrow stromal cells with Feridex and in vivo tracking after autologous transplantation with MRI
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1671-8925.2005.02.002
- Key Word:
- 骨髓细胞;干细胞;移植,自体;磁共振成像;菲立磁;超顺磁性氧化铁
Abstract： 目的初步探索利用菲立磁(Feridex)和转染试剂体外磁性标记大鼠骨髓基质细胞这一方法的可行性,为将来临床上应用磁共振成像(MRI)追踪标记细胞奠定基础.方法无菌条件下行股骨取骨髓,梯度密度离心法分离获取大鼠骨髓基质细胞,使用"Feridex-多聚赖氨酸复合物(FE-PLL)"标记骨髓基质细胞,采用普鲁士蓝染色、电镜和台盼蓝排除实验等方法鉴定FE-PLL标记大鼠骨髓基质细胞的效率和细胞的活力,并对FE-PLL标记骨髓基质细胞的增殖和分化能力进行评估.将FE-PLL标记和未标记后的骨髓基质细胞分别移植入大鼠左右侧尾壳核脑内,细胞移植后1、4、7周应用MRI对脑内移植的细胞进行活体示踪,最后利用组织切片进行普鲁士蓝染色.结果菲立磁可以高效率地标记骨髓基质细胞,标记效率在99%左右.普鲁士蓝染色显示FE-PLL标记骨髓基质细胞胞质内出现细小的蓝色铁颗粒.电镜结果显示FE-PLL标记的骨髓基质细胞胞质内含有许多包裹铁颗粒的囊泡.与正常未标记的细胞相比较,FE-PLL标记对骨髓基质细胞的活力、增殖和分化等能力没有明显的影像.MRI检查发现脑内移植的标记细胞在磁共振上呈明显的低信号改变,未标记细胞侧脑组织无明显的低信号改变,与组织学切片结果基本相一致.结论以上结果提示菲立磁可以用来体外标记骨髓基质细胞,利用MRI技术可以对脑内移植后的标记细胞进行初步的活体追踪.
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