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Cytogenetic analysis on bone marrow-derived neural stem cells cultured in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-8925.2005.01.005
- Key Word:
- 骨髓细胞;干细胞;细胞遗传学分析;核型分析
Abstract： 目的评价体外培养的成人骨髓源性神经干细胞的遗传稳定性.方法制备骨髓源性神经干细胞的染色体标本,以常规染色和G显带进行染色体的核型分析.结果体外培养的成人骨髓源性神经干细胞的染色体数目为正常人类的46条染色体,未见非整倍体染色体像;G显带图像核型分析显示其为二倍体正常核型,染色体中未发现有缺失、倒位、易位、双着丝粒和环状染色体等结构异常.结论体外培养分化形成的骨髓源性神经干细胞保持了遗传学上的稳定性,为其进一步的临床应用提供了实验依据.
- [1]李钢,柯以铨.神经干细胞的来源[J].2003,(5).
- [2]戴宜武,徐如祥,赵春平,等.骨髓基质细胞源神经干细胞自体移植治疗脑干损伤初步观察[J].2003,(1).
- [3]姜晓丹,徐如祥,张世忠,等.成人骨髓源性神经干细胞诱导分化实验研究[J].2002,(11).
- [4]钟池,钟春玖,罗玉敏,等.骨髓基质细胞静脉移植治疗大鼠短暂性局灶性脑缺血[J].2004,(2).
- [5]Okano H.Stem cell biology of the central nervous system.[J].2002,69(6).
- [6]Krause DS.Plasticity of marrow-derived stem cells.[J].2002,9(11).
- [7]Temple S.Stem cell plasticity--building the brain of our dreams.[J].2001,2(7).
- [8]Bianco P,Riminucci M,Gronthos S,etc.Bone marrow stromal stem cells: nature, biology, and potential applications.[J].2001,19(3).
- [9]Li Y,Chopp M,Gautam SC,etc.Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat.[J].2003,73(6).
- [10]刘权章.人类染色体方法学[M].1992.