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Expression of NF-κBp65 in focal cerebral ischemic reperfusion
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.05.004
- Key Word:
- 脑缺血再灌注;NF--κBp65;免疫组化;RT-pCR;测序
Abstract： 目的确定NF-κBp65在局灶性脑缺血再灌注后的空间及时间分布特征并测序.方法采用免疫组化、RNA提取与RT-pCR技术及测序方法.结果在脑缺血再灌注后不同时程及缺血边缘可见不同形态特征的神经元,在血脑屏障和血管周围的中性粒细胞也可见NF-κBp65阳性反应.脑缺血2 h再灌注后24 h缺血侧NF-κBp65 mRNA RT-PCR的PCR产物相对量明显增高.结论NF-κBp65激活在局灶性脑缺血再灌注后的半暗区和血脑屏障中起重要作用.在恰当的时间窗内针对NF-κBp65的治疗可能是潜在的治疗途径.
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