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Feasibility of tracing the bone marrow-derived stem cells in brain tissue with retrovirus pLXSN-GFP
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.05.003
- Key Word:
- 绿色荧光蛋白;骨髓源干细胞;嗅球;脑干网状结构
Abstract： 目的探讨应用逆转录病毒pLXSN-GFP在脑组织追踪骨髓源干细胞的可行性.方法应用逆转录病毒pLXSN-GFP标记Sprague-Dawley大鼠骨髓源干细胞,经尾静脉植入C57BL/6小鼠.在植入后4周和8周取材.用荧光显微镜和激光共聚焦显微镜观察绿色荧光阳性细胞,同时用GFP抗体的荧光免疫组织化学方法判断绿色荧光的来源.结果移植骨髓源干细胞后4周和8周,在嗅球和脑干网状结构均可见绿色荧光阳性细胞,GFP抗体的荧光免疫组织化学方法证实绿色荧光的确来源于GFP的表达.结论逆转录病毒pLXSN-GFP可用以标记并在脑组织追踪骨髓源干细胞.
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