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HPLC determination of amino acid neurotransmitter in cerebrospinal fluid of PVS patients
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.04.004
- Key Word:
- 高效液相色谱分析;持续性植物状态;脑脊液;氨基酸;神经递质
Abstract： 目的探讨HPLC法检测脑脊液中氨基酸类物质的方法,观察PVS患者脑脊液中氨基酸类神经递质的变化.方法28例PVS患者与15例对照病人分别采集脑脊液,经过预处理后运用OPA柱前衍生化法分别检测谷氨酸(glutamicacid,Glu)、天冬氨酸(aspartic acid,Asp)、丙氨酸(alanine,Ala)、甘氨酸(glycine,Gly).结果本实验条件下,4种氨基酸在25 min内得以较好分离.峰面积与氨基酸浓度的线性关系良好.PVS组4种递质水平均高于对照组,其中Ala的差异有统计学意义(P＜0.05).结论HPLC法检测脑脊液中氨基酸类物质方法简便易行,准确度高.PVS患者脑脊液中Ala水平显著升高.
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