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Expression of cyclooxygenase-2 after focal cerebral ischemic reperfusion in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.03.003
- Key Word:
- 脑缺血再灌注;COX-2;免疫组化;RT-PCR;测序
Abstract： 目的确定COX-2免疫组化的空间分布特点,以及mRNA RT-PCR产物表达的时间分布特征并测序.方法采用石蜡包埋的免疫组化技术,脑组织中RNA提取、RT-PCR技术及测序分析.结果局灶性脑缺血再灌注后COX-2阳性神经元多位于梗死边缘区,另外血脑屏障也可见COX-2免疫反应阳性.COX-2 mRNA RT-PCR的PCR产物相对量,在脑缺血再灌注后15～24 h明显增高(P＜0.01).结论COX-2介导着脑缺血再灌注后半暗带和血脑屏障的存活和死亡,在恰当的时间窗内针对COX-2的治疗可能对脑保护是有效的.
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