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Influences of inhibitors of nitric oxide synthases on motor functional recovery after spinal cord injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.03.001
- Key Word:
- 一氧化氮合酶;脊髓损伤;运动功能
Abstract： 目的观察诱导型和神经型一氧化氮合酶(iNOS,nNOS)抑制剂对大鼠脊髓损伤(SCI)后运动功能的影响和机理.方法大鼠脊髓压迫伤后分别给予iNOS和nNOS抑制剂-氨基胍(AG)和7-硝基吲唑(7-NI)进行治疗,24 h后用分光光度法测定组织中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性,72 h后用流式细胞仪检测神经细胞凋亡情况,4周后用电生理和动物行为学等指标评价运动功能的恢复情况.结果AG和7-NI均可以抑制组织中的NO含量,并使NOS活性下降,同时降低神经细胞的凋亡比率,对运动功能的恢复前者优于后者.结论脊髓损伤后应用NOS抑制剂可以使伤后运动功能得到改善,AG的作用似乎更明显,提示iNOS活性变化可能对脊髓损伤的恢复更具决定作用.
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