You Position: Home > Paper
The intermolecular forces between acetylcholinesterases and reactivators
( views:251, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-8925.2004.01.003
- Key Word:
- 原子力显微技术;乙酰胆碱酯酶;分子间作用力;重活化剂
Abstract： 目的研究乙酰胆碱酯酶(AChE)重活化剂与正常和中毒AChE分子间作用力.方法原子力显微技术(AFM).结果PAM-Cl、OBD和HI-6与正常AChE粘附力分别为28±2 pN、40±3 pN和52±6 pN,与沙林中毒AChE间粘附力可分别增加至830±120 pN、1099±143 pN和1544±98 pN,为正常AChE的30、27和30倍;与VX中毒AChE间粘附力可分别增加至928±122 pN、1375±143 pN和1288±11.2 pN,为中毒前的25、30和26倍;对梭曼中毒AChE没有重活化作用的PAM-Cl和OBD与梭曼中毒AChE粘附力没有明显增加;对梭曼中毒酶有重活化作用的HI-6与梭曼中毒AChE间粘附力为854±86 pN,为中毒前16倍.结论重活化剂-中毒AChE分子间粘附力与药物重活化作用一致,是重活化作用的力学基础.
- [1]张英鸽,饶曼人.原子力显微技术的基本原理及药理学应用[J].2000,(6).
- [2]张英鸽,白春礼,王琛,等.用原子力显微技术研究乙酰胆碱与乙酰胆碱酯酶之间的作用力[J].1999,(3).
- [3]张英鸽,王琛,赵德禄,等.乙酰胆碱酯酶分子的原子力显微成像[J].1999,(1).
- [4]G. Binnig,C. F. Quate,Ch. Gerber.Atomic Force Microscope[J].1986,56(9).
- [5]张英鸽.用原子力显微技术测定生物分子之间的相互作用力[J].1998,(4).
- [6]Chen W,Delu Z,Yingge Z,etc.Force spectroscopy between acetylcholinesterase molecule and its natural substrate to study the effects of inhibitors and reactivators on enzyme activity.[J].1999,65(21).
- [7]Charles B. Millard,Gertraud Koellner,Arie Ordentlich,etc.Reaction Products of Acetylcholinesterase and VX Reveal a Mobile Histidine in the Catalytic Triad[J].1999,121(42).
- [8]张英鸽,赵德禄.原子力显微技术成像在生物医学中的应用[J].1999,(1).
- [9]Cutler NR,Sramek JJ,Krall WJ.Cholinesterase inhibitors: a therapeutic strategy for Alzheimer disease.[J].1999,33(4).
- [10]孙曼霁,高天栋,邢志勇,等.中国丁氏双鳍电鳐乙酰胆碱酯酶的制备亲和层析[J].1985,(2).
- [11]Han T.,Beebe TP.,Williams JM..Chemical Bonds Studied with Functionalized Atomic Force Microscopy Tips[J].1995,307(2).
- [12]Murray TK,Misra A,Cross AJ,etc.A comparative study in rats of the in vitro and in vivo pharmacology of the acetylcholinesterase inhibitors tacrine, donepezil and NXX-066.[J].1999,38(1).
- [13]Michel Grandbois,Martin Beyer,Matthias Rief,etc.How Strong Is a Covalent Bond?[J].1999,283 (5408).