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A new method of rapid isolation of adult human myoblast
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROMEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1671-8925.2003.06.002
- Key Word:
- 成肌细胞;分离;增殖;分化
Abstract： 目的建立快速分离成人成肌细胞的新方法.方法改进成肌细胞分离方法,即缩短分离时间并增加分离细胞数.结果(1)新分离法组织离体到细胞接种所需的时间为2.1±0.52 h,与传统分离法6.8±0.67 h比较,细胞分离所需时间显著缩短(P＜0.01).(2)新分离法每克组织分离获取的细胞数为(8.2±.88)×106较传统分离法(3.0±1.07)×106显著增加(P＜0.01),且纯化后的细胞Desmin(+)率＞97%.(3)新分离法和传统分离法分离细胞在生长液中生长的倍增时间分别为25.57h和32.39h,即新方法使成肌细胞倍增时间提前了约7h.(4)新方法和旧方法获取细胞的成肌分化率分别为90.8±1.14%和90.4±0.85%,无显著差异(P＞0.05).结论新分离法能快速分离获取纯度高倍增快的成人成肌细胞.
- [1]Z, Yablonka-Reuveni,L S, Quinn,M, Nameroff.Isolation and clonal analysis of satellite cells from chicken pectoralis muscle.[J].1987,119(1).
- [2]T A, Rando,H M, Blau.Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy.[J].1994,125(6).
- [3]Cummins J,Ikezawa M,Pruchnic R,etc.Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration.[J].2002,157(5).
- [4]C, Richler,D, Yaffe.The in vitro cultivation and differentiation capacities of myogenic cell lines.[J].1970,23(1).